CCR5 antibody (2nd Extracellular Loop)

Catalog No. ABIN7886248

The Rabbit Polyclonal anti-CCR5 antibody (ABIN7886248) specifically detects CCR5 in WB, FACS and LCI. The antibody is reactive with Human samples.

Quick Overview for CCR5 antibody (2nd Extracellular Loop) (ABIN7886248)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CCR5 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 178-192

Purpose: A Rabbit Polyclonal Antibody to CCR5 Chemokine Receptor

Cross-Reactivity (Details): Not recommended for use with rat and mouse samples.

Predicted Reactivity: Human only

Characteristics: Anti-Human CCR5 (extracellular) Antibody (ABIN7043034, ABIN7044174 and ABIN7044175) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize CCR5 from human samples only. The antibody is unlikely to recognize the receptor from mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CSSHFPYSQYQFWKN, corresponding to amino acid residues 178-192 of human CCR5

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5

Background:

Synonyms: C-C chemokine receptor type 5, C-C CKR-5, CHEMR13, HIV-1 fusion coreceptor, CD195

Description: CCR5 protein is a G-protein coupled receptor (GPCR) responsible for regulating host immune responses and functions against pathogens. The protein plays a key role in transferring immune cells to inflammatory sites and injury. CCR5 is also considered to be an anchor protein for R5-tropic strains of HIV-1 (Human Immunodeficiency Virus-1) to infect immune cells. Thus, CCR5 contributes to the development of AIDS (Acquired Immunodeficiency Syndrome).The receptor is widely expressed in a variety of immune cells involved in the inflammatory responses such as macrophages, monocytes, T cells and dendritic cells1,2.CCR5 is the receptor for the CC ligand 3 (CCL3), CC ligand 4 (CCL4), and CC ligand 5 (CCL5) chemokine ligands. CCR5 and its ligands are upregulated during HIV infection.Studies have shown that lack of homozygosity of CCR5Δ32 variant, a 32 base-pair deletion of the CCR5's gene coding region, is associated with preventing the risk of HIV-1 infection2,3.

Gene ID: 1234

NCBI Accession: NM_000579

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size