CX3CR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886259

This Rabbit Polyclonal antibody specifically detects CX3CR1 in WB, FACS, IHC and LCI. It exhibits reactivity toward Mouse.

Quick Overview for CX3CR1 antibody (Extracellular, N-Term) (ABIN7886259)

Target: CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CX3CR1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-CX3CR1 Antibody

Binding Specificity: AA 7-20, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to CX3CR1 Receptor

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Rat - identical

Characteristics: A Rabbit Polyclonal Antibody to CX3CR1 Receptor

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ELDLENFEYDDSAE, corresponding to amino acid residues 7- 20 of mouse CX3CR1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235074)

Blocking Peptide: (ABIN7235074)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CX3CR1

Target: CX3CR1 (Chemokine (C-X3-C Motif) Receptor 1 (CX3CR1))

Alternative Name: CX3CR1

Background:

Synonyms: Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Description: CX3C-chemokine receptor 1 (CX3CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors1.CX3CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX3C chemokine ligand 1, (CX3CL1). The major role of CX3CR1 in immune cells is to recognize and enter inflamed tissue according to CX3CL1 gradient and initiate the innate immune system2. The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)3. Expression and interaction of CX3CR1 with CX3CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).Both CX3CL1 and CX3CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX3CL1/CX3CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX3CL1/CX3CR1 signaling is associated with Alzheimer's Disease5. In the liver, CX3CR1 is expressed in monocytes, CD8+ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites4.

Gene ID: 13051

NCBI Accession: NM_001337

UniProt: Q9Z0D9

Pathways: Cellular Response to Molecule of Bacterial Origin