CLIC1 antibody (C-Term, Intracellular)

Catalog No. ABIN7886226

This Rabbit Polyclonal antibody specifically detects CLIC1 in WB, IF and IC. It exhibits reactivity toward Human.

Quick Overview for CLIC1 antibody (C-Term, Intracellular) (ABIN7886226)

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLIC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-CLIC1 Antibody

Binding Specificity: AA 195-208, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CLIC1 Channel

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-CLIC1 Antibody (ABIN7043056 and ABIN7044139) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:200

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235102)

Blocking Peptide: (ABIN7235102)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CLIC1

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Alternative Name: CLIC1

Background:

Synonyms: Cl- intracellular channel 1, Nuclear chloride ion channel 27, NCC27, Chloride channel ABP

Description: Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1.CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon4. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration5.

Gene ID: 1192

NCBI Accession: NM_001288

UniProt: O00299