CCKBR antibody (Extracellular)

Catalog No. ABIN7043059

This Rabbit Polyclonal antibody specifically detects CCKBR in WB, IHC, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for CCKBR antibody (Extracellular) (ABIN7043059)

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCKBR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CCKBR Antibody

Binding Specificity: AA 39-53, Extracellular

Purpose: A Rabbit Polyclonal Antibody to the Cholecystokinin B Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 13,Rat - 14,15 amino acid residues identical

Characteristics: Anti-CCKBR (extracellular) Antibody (ABIN7043059, ABIN7044183 and ABIN7044184) is a highly specific antibody directed against an epitope of the mouse cholecystokinin B receptor. The antibody can be used in western blot, indirect flow cytometry, and immunohistochemistry applications. It has been designed to recognize CCKBR from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CETPRIRGTGTRELE, corresponding to amino acid residues 39-53 of mouse Gastrin/cholecystokinin type B receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100-1:200

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IFC

Negative Control: (ABIN7235114)

Blocking Peptide: (ABIN7235114)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Alternative Name: CCKBR

Background: Cholecystokinin B receptor, Gastrin receptor, CCK2R, GASR,Cholecystokinin B receptor (CCKBR), also known as the Gastrin receptor and CCKR2, belongs to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily, and is one of the two receptors that mediates the effects of the cholecystokinin (CCK) and gastrin peptides1,2.CCK and gastrin are related linear peptides that occur in different forms but share the last five C-terminal amino acid residues. CCK regulates several nutritional-related activities such as stimulation of pancreatic exocrine secretion or the regulation of intestinal transit, while gastrin's main function is the stimulation of gastric acid secretion1,2.Both CCKBR and the other CCK receptor (CCKAR or CCK1R) are coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), and intracellular Ca2+mobilization.CCKBR was originally identified in the brain (and hence its name, type B for Brain) where it is widely distributed, notably in the cerebral cortex and striatum. In the periphery, CCKBR is most notably expressed in acid secreting cells in the mucosa of the stomach. The tissue distribution of CCKBR corresponds to the proposed roles of the receptor which include anxiety, pain perception, gastric acid secretion, and growth and differentiation of the gastric mucosa1,2.

Alternative names: CCKBR, Cholecystokinin B receptor, Gastrin receptor, CCK2R, GASR

Gene ID: 12426

NCBI Accession: NM_176875

UniProt: P56481

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour