CHRM1 antibody (3rd Intracellular Loop)

Catalog No. ABIN7886549

The Rabbit Polyclonal anti-CHRM1 antibody is suitable to detect CHRM1 in samples from Human. It has been validated for WB, IHC, IF and IC.

Quick Overview for CHRM1 antibody (3rd Intracellular Loop) (ABIN7886549)

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-CHRM1 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 227-353

Purpose: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: mouse - 124, rat - 123,Macaca mulatta - 125,127 amino acid residues identical, gerbil - 123, pig - 124

Characteristics: Anti-CHRM1 Antibody is directed against the 3rd intracellular loop of the human M1 muscarinic receptor. Anti-CHRM1 Antibody (ABIN7043060, ABIN7044580 and ABIN7044581) can be used in western blot analysis, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize M1 from human, mouse and rat samples.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogen: GST fusion protein with the sequence GSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRK, corresponding to amino acid residues 227-353 of human M1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7235983)

Blocking Peptide: (ABIN7235983)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CHRM1

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Alternative Name: CHRM1

Background:

Synonyms: Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1

Description: The action of the neurotransmitter acetylcholine (ACh) is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named M1-M5.1-2The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues.1-2Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.3-4 The M1 receptors are the most abundant muscarinic subtype in the cortex and striatum. M1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of ACh from the nerves.5-6The M1, M3 and M5 receptors, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the M1, M3 and M5 receptors is an essential element for the anti-apoptotic response of those receptors.7

Gene ID: 1128

NCBI Accession: NM_000738

UniProt: P11229

Pathways: Synaptic Membrane