Contactin 1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886682

This Rabbit Polyclonal antibody specifically detects Contactin 1 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for Contactin 1 antibody (Extracellular, N-Term) (ABIN7886682)

Target: Contactin 1 (CNTN1)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Contactin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Contactin 1 Antibody

Binding Specificity: AA 947-961, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Contactin-1 (extracellular)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-Contactin-1/CNTN1 (extracellular) Antibody (ABIN7043083, ABIN7044738 and ABIN7044739) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used western blot and immunohistochemistry applications. It has been designed to recognize CNTN1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RPDGQHDGKLFSTHK, corresponding to amino acid residues 947 - 961 of rat Contactin-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7235161)

Blocking Peptide: (ABIN7235161)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Contactin 1

Target: Contactin 1 (CNTN1)

Alternative Name: CNTN1

Background:

Synonyms: Neural cell surface protein F3, Glycoprotein GP135, CNTN1

Description: Contactin-1 (CNTN1), also known as F3/Contactin a cell adhesion molecule, is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein involved in neuronal development, synaptic maintenance and organization of neuronal networks. CNTNs are a subpopulation of molecules belonging to the immunoglobulin (Ig) superfamily, which includes six members: CNTN1, TAG-1/CNTN2, BIG-1/CNTN3, BIG-2/CNTN4, NB-2/CNTN5 and NB-3/CNTN61,2.In mammals, contactin-1 is mostly localized in the peripheral and central neural system. It is highly expressed at the axonal level and mediates various functions in both glial and neuronal cells, it is involved in neuronal and glial development and differentiation, myelination, synaptogenesis and synaptic function1.Contactin-1 expression profile differs in various brain regions and, within each area, it changes depending upon the cell type. For example, in the cerebellar cortex contactin-1 is predominant in granule cells in earlier developmental stages, whereas its activation is delayed in Purkinje neurons. In the hippocampus, it is detected on pyramidal neurons of CA1-CA3 fields and in granule layers of Dentate Gyrus (DG) where its expression persists in postnatally developed neurons. Hippocampal contactin-1 is highly expressed in the developing hippocampus, it progressively increases during postnatal life, but it declines during aging, paralleling cognitive impairment. This suggests that contactin-1 might play a role in memory processes, in line with studies indicating its involvement in neurotransmitter release and some forms of plasticity1.The role of contactin-1 in neurotransmitter release has been linked with its capability to regulate synaptic vesicle exocytosis. Contactin-1 overexpression had been shown to induce an increase of cAMP-Responsive Element Binding (CREB) phosphorylation, enhanced hippocampal-dependent LTP and memory especially in 12-months-old mice, where neurogenesis is particularly evident. Also, contactin-1 overexpression counteracts apoptosis, and induces an increase of the antiapoptotic- and neuroplasticity-pathway BDNF/CREB/Bcl-21,3.Contactin-1 plays an important role in cancer progression. The expression of contactin-1 is upregulated in primary lesions, and its expression level correlates with tumor metastasis in cancer patients. Studies have demonstrated that contactin-1 also plays a key role in diseases not related to the nervous system, and its most notable function is its participation in cancer progression, in cancers such as esophageal squamous cell carcinoma (ESCC), GC, lung adenocarcinoma, oral squamous cell carcinoma (OSCC), hepatocellular carcinoma and prostate cancer2,3.

Gene ID: 117258

NCBI Accession: NM_001843

UniProt: Q63198