CNGA1 antibody (Intracellular)

Catalog No. ABIN7043089

Product Details anti-CNGA1 Antibody

Target: CNGA1 (Cyclic Nucleotide Gated Channel alpha 1 (CNGA1))

Binding Specificity: AA 41-54, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNGA1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to CNGA1 Channel

Specificity: Intracellular, N-terminus

Predicted Reactivity: 14 amino acid residues identical,Mouse - 13, human - 9

Characteristics: Anti-CNGA1 Antibody (ABIN7043089 and ABIN7044984) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CNGA1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NDNGSLSEESENED, corresponding to amino acid residues 41-54 of rat CNGA1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235181)

Blocking Peptide: (ABIN7235181)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CNGA1

Target: CNGA1 (Cyclic Nucleotide Gated Channel alpha 1 (CNGA1))

Alternative Name: CNGA1 (CNGA1 Products)

Background: Cyclic nucleotide-gated cation channel 1, CNG channel alpha-1, CNG1, CNCG1, Rod photoreceptor cGMP-gated channel subunit alpha, RCNC1, RP49,Cyclic nucleotide-gated (CNG) channels belong to the superfamily of voltage-gated ion channels. Although permeable to various ions such as the monovalent Na+ and K+ ions, and the divalent Ca2+ ion, they are gated by the intracellular binding of the cyclic nucleotides cAMP and cGMP and not by voltage per se (CNGs bind preferably to cGMP)1.Six channels form this subfamily: The A subunit (CNGA1-4) and the B subunit (CNGB1 and CNGB3). Functional entities are formed by the assembly of four subunits. Each subunit consists of six membrane spanning domains, where the fourth transmembrane domain is highly positively charged, a pore domain between transmembrane domains five and six, and intracellular N- and C-termini. The cyclic nucleotide binding domain is located in the C-terminal region and is responsible for the channel gating upon cyclic nucleotide binding1.These channels are highly expressed in retinal photoreceptors and olfactory neurons where their role has been extensively studied2. CNG channels have also been detected in brain, testis and kidney although their role in these tissues has yet to be unraveled2. When heterologously expressed all subunits with exception of CNGA4, CNGB1 and CNGB3 can form functional homomeric channels1. In rod photoreceptors, functional channels are composed of three CNGA1 subunits and one CNGB1 subunit3.Mutations in either of CNGA1 or CNGB1 genes are responsible for the onset of retinitis pigmentosa, a progressive degeneration of rod and cone receptors which is presented in part by night blindness3-4. As no knock out CNGA1 model systems exist, a transgenic mice line with lowered CNGA1 protein levels demonstrated symptoms which are reminiscent of retinitis pigmentosa, in agreement with the fact that a lack of CNGA1 is responsible for retinitis pigmentosa4.

Alternative names: CNGA1, Cyclic nucleotide-gated cation channel 1, CNG channel alpha-1, CNG1, CNCG1, Rod photoreceptor cGMP-gated channel subunit alpha, RCNC1, RP49

Gene ID: 85259

NCBI Accession: NM_000087

UniProt: Q62927

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction, Asymmetric Protein Localization