DPP6 antibody (Extracellular)

Catalog No. ABIN7043098

Product Details anti-DPP6 Antibody

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Binding Specificity: AA 414-426, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPP6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Dipeptidyl Peptidase 6

Specificity: Extracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-DPP6 (extracellular) Antibody (ABIN7043098, ABIN7045042 and ABIN7045043)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize DPP6 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KKHEDESEAWLHR, corresponding to amino acid residues 414-426 of human DPP6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Negative Control: BLP-PC146

Blocking Peptide: BLP-PC146

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for DPP6

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Alternative Name: DPP6 (DPP6 Products)

Background: Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-like protein 6, DPP VI, DPPX, VF2,Dipeptidyl-peptidase 6 (DPP6) is a type II membrane glycoprotein1 which is a part of the dipeptydil aminopeptidase-like proteins (DPPLs) subfamily. It is an auxiliary glycoproteinic subunit of voltage-dependent K+ channel 4 (KV4.x), that has a short cytoplasmic domain, a long extracellular domain and a transmembrane domain. It regulates sub-threshold, A-type K+ currents, and is important for the enhancement and localization of KV4.2 channels2.DPPLs are a subgroup of the larger prolyl oligopeptidase family. A loss-of-function mutation at their enzymatic serine renders them incapable of cleaving peptides. Notable DPPLs include DPP8, DPP9, and DPPIV - the latter is known to regulate several key functions in varied systems, ranging from immune to CNS1,3.Alternative mRNA splicing of the DPP6 gene (part of the di-genic DPLP group that also consists DPP10) gives rise to several isoforms that differ in their N-terminal (DPP6a, DPP6K, DPP6S, DPP6L DPP6E)4.DPP6 proteins are widely expressed throughout both of the nervous systems - albeit much more predominantly in the CNS - and are known to play a major role in neurogenesis and neurodevelopment5.Deletion mutations and haplo-insufficiency of the DPP6 gene has been shown to be associated with autism spectrum disorder, Gilles de la Tourette syndrome, dominant microcephaly and mental retardation5,6. Endometriosis - a condition characterized by extra-uteral endometrial glands that affects one tenth of fertile women population - has been linked with DPP6 in genome wide associations studies (GWASs)7.

Alternative names: DPP6, Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-like protein 6, DPP VI, DPPX, VF2

Gene ID: 1804

NCBI Accession: NM_130797

UniProt: P42658