DRD5 antibody (Extracellular)

Catalog No. ABIN7043111

Product Details anti-DRD5 Antibody

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Binding Specificity: AA 199-211, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DRD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to D5 Dopamine Receptor

Specificity: 2nd extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Mouse - 12,13 amino acid residues identical

Characteristics: Anti-D5 Dopamine Receptor (extracellular) Antibody is directed against an epitope located at the 2nd extracellular loop of the rat D5 dopamine receptor. Anti-D5 Dopamine Receptor (extracellular) Antibody (ABIN7043111, ABIN7044234 and ABIN7044235) can be used in western blot analysis and immunohistochemical applications, and recognizes DRD5 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EEGWELEGRTENC, corresponding to amino acid residues 199-211 of rat DRD5 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235250)

Blocking Peptide: (ABIN7235250)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for DRD5

Target: DRD5 (Dopamine Receptor D5 (DRD5))

Alternative Name: DRD5 (DRD5 Products)

Background: DRD5, D(1B) dopamine receptor,The D5 dopamine receptor (D5 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and Schizophrenia.1All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1 The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1The D5 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex, hippocampus and striatum. In the periphery, the D5 receptor has been localized in the adrenal cortex, kidney and intestinal tract.The exact physiological function of the D5 receptor subtype remains poorly understood. Studies with D5 receptor knockout mice have shown no overt alterations in locomotor or cognitive functions. However, knockout mice do develop severe hypertension suggesting a role of the D5 receptor in the modulation of neuronal pathways regulating blood pressure responses.2,3

Alternative names: D5 Dopamine Receptor, DRD5, D(1B) dopamine receptor

Gene ID: 25195

NCBI Accession: NM_000798

UniProt: P25115

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity