EPH Receptor A3 antibody (Extracellular)

Catalog No. ABIN7043123

The Rabbit Polyclonal anti-EPH Receptor A3 antibody has been validated for WB, IHC, FACS, IF and LCI. It is suitable to detect EPH Receptor A3 in samples from Human.

Quick Overview for EPH Receptor A3 antibody (Extracellular) (ABIN7043123)

Target: EPH Receptor A3 (EPHA3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-EPH Receptor A3 Antibody

Binding Specificity: AA 488-500, Extracellular

Purpose: A Rabbit Polyclonal Antibody to EphA3 Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-EphA3 (extracellular) Antibody (ABIN7043123, ABIN7044245 and ABIN7044246) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize EphA3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RARGTNVTISSLK, corresponding to amino acid residues 488-500 of human EphA3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235282)

Blocking Peptide: (ABIN7235282)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for EPH Receptor A3

Target: EPH Receptor A3 (EPHA3)

Alternative Name: EPHA3

Background: Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4,Eph receptors are the largest family of receptor tyrosine kinases (RTKs). Based on sequence similarity and the type of ephrin ligands that they bind, they are classified into A and B sub-classes. EphA receptors bind ephrin-A ligand while EphB bind ephrin-B ligand.Eph receptors play an important role during embryonic development and are involved in several processes including cell adhesion, cell migration, and tissue boundary formation. Eph receptors are typically expressed at low levels in normal adult tissues1,2.EphA3 (Ephrin type-A receptor 3) structure includes a ligand-binding domain (LBD) at the N-terminus followed by a cysteine-rich region and two fibronectin III repeats. The receptor-binding domain of the ephrins is connected through a short linker segment to a GPI-anchor or a transmembrane segment3.EphA3 was first identified as a surface antigen on pre-B lymphoblastic leukemia cells. High EphA3 levels have been detected in a number of hematological cancers and solid tumors such as lung cancers, melanomas, gastric carcinoma, leukemia, in B and T cell malignancies and in glioblastoma multiforme. In addition, high levels of EphA3 are implicated in lymph node metastasis, advanced stages of colorectal cancer, and prostate cancer1,3,4.

Alternative names: EphA3, Ephrin type-A receptor 3, EPH-like kinase 4, EK4, Tyrosine-protein kinase TYRO4

Gene ID: 2042

NCBI Accession: NM_005233

UniProt: P29320

Pathways: RTK Signaling, Regulation of Cell Size