EPH Receptor A4 antibody (Extracellular)

Catalog No. ABIN7043124

Product Details anti-EPH Receptor A4 Antibody

Target: EPH Receptor A4 (EPHA4)

Binding Specificity: AA 485-499, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal antibody to EphA4 (extracellular).

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: A rabbit polyclonal antibody to EphA4 (extracellular).

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ERSYRIVRTAARNTD, corresponding to amino acid residues 485 - 499 of human EPHA4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235286)

Blocking Peptide: (ABIN7235286)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for EPH Receptor A4

Target: EPH Receptor A4 (EPHA4)

Alternative Name: EPHA4 (EPHA4 Products)

Background: Ephrin Type-A Receptor 4, EPH-Like Kinase 8, EPHA4,EphA4 is a member of the Eph receptor tyrosine kinase family.Eph receptors are subdivided into two subclasses, termed EphA and EphB, based on sequence similarity and their preference for binding a particular subclass of ephrins. The domain organization of Eph receptors contains a globular ligand binding domain (LBD), a cysteine-rich region, and two fibronectin type III domains (FN1 and FN2). It also contains a transmembrane (TM) helix, and an intracellular part consisting of a juxtamembrane (JM) region and a tyrosine kinase domain. Eph kinase activity is auto-inhibited through interaction with its own JM region, this auto inhibition is released by phosphorylation1.Interactions of Eph receptors with their ligands, ephrins, at cell-cell interfaces promote a variety of cellular responses, including repulsion, attraction and migration2.EphA4 is activated by all members of the ephrin-A ligand family and most members of the ephrin-B family1. Upon ligand binding it activates pathways that regulate the formation of the axon tract and cortical network as well as radial migration of cortical neurons in the cortex3.EphA4 is expressed in the neural ectoderm at embryonic day 8 and then in various cell types throughout the cortex during subsequent stages of neurodevelopment4, its major effect on cell-cell interfaces makes this receptor highly important for the study of cellular regeneration and various types of disease.

Alternative names: Ephrin Type-A Receptor 4, EPH-Like Kinase 8, EPHA4

Gene ID: 2043

NCBI Accession: NM_001304536

Pathways: RTK Signaling