Ephrin A1 antibody (Extracellular)

Catalog No. ABIN7043128

Product Details anti-Ephrin A1 Antibody

Target: Ephrin A1 (EFNA1)

Binding Specificity: AA 150-164, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Ephrin-A1

Specificity: Extracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 13,15 amino acid residues identical

Characteristics: Anti-Ephrin-A1 (extracellular) Antibody is directed against an extracellular epitope of human Ephrin-A1. Anti-Ephrin-A1 (extracellular) Antibody (ABIN7043128, ABIN7044255 and ABIN7044256) can be used in western blot and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting Ephrin-A1 in living cells. It has been designed to recognize Ephrin-A1 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KITHSPQAHDNPQEK, corresponding to amino acid residues 150-164 of human Ephrin-A1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7235302)

Blocking Peptide: (ABIN7235302)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Ephrin A1

Target: Ephrin A1 (EFNA1)

Alternative Name: EFNA1 (EFNA1 Products)

Background: EPH-related receptor tyrosine kinase ligand 1, LERK1, EFNA1,Ephrin-A1, a ligand of type-A Eph receptor tyrosine kinases and the Eph/ephrin system, has been shown to play important roles in the nervous system, tissue patterning and cell adhesion through receptor-ligand interactions and intracellular signaling1. Ephrins can be divided into two subclasses: ephrin A and ephrin B. Ephrin A ligands (ephrin A1-A5) are anchored to the cell surface via a glycosylphosphatidylinositol (GPI) anchor and bind to Eph A receptors (Eph A1-A8), whereas ephrin B ligands (ephrin B1-B3) have transmembrane and cytoplasmic domains and interact with Eph B receptors (Eph B1-B6)2.Eph receptors and ephrins are highly expressed in the brain and in the developing nervous system, where they have well-known roles in the establishment of neuronal connectivity by guiding axons to the appropriate targets and regulating the formation of synaptic connections. Ephrins are expressed in numerous regions of the vertebrate embryo including the ectoderm, mesoderm, and endoderm3.Ephrin (Eph) receptors are frequently overexpressed in a variety of human malignant tumors, and are associated with tumor growth, invasion, metastasis and angiogenesis. Recent studies using clinical samples showed that ephrin-A1 expression was up-regulated in colorectal cancer and hepatocellular carcinoma and positively correlated with a poor prognostic value4.

Alternative names: Ephrin-A1, EPH-related receptor tyrosine kinase ligand 1, LERK1, EFNA1

Gene ID: 1942

NCBI Accession: NM_004428

UniProt: P20827

Pathways: RTK Signaling