Ephrin A2 antibody (Extracellular)

Catalog No. ABIN7886304

The Rabbit Polyclonal anti-Ephrin A2 antibody is suitable to detect Ephrin A2 in samples from Mouse. It has been validated for WB, IF, FACS, IHC and LCI.

Quick Overview for Ephrin A2 antibody (Extracellular) (ABIN7886304)

Target: Ephrin A2 (EFNA2)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-Ephrin A2 Antibody

Binding Specificity: AA 71-84, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Ephrin-A2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-Ephrin-A2 (extracellular) Antibody (ABIN7043129, ABIN7044257 and ABIN7044258) is a highly specific antibody directed against an epitope of mouse EFNA2. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize EFNA2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235305)

Blocking Peptide: (ABIN7235305)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Ephrin A2

Target: Ephrin A2 (EFNA2)

Alternative Name: EFNA2

Background:

Synonyms: EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2

Description: Eph receptor tyrosine kinases family and their ligands, ephrins, are membrane proteins that primarily regulate cell to cell repulsion and cell adhesion and movement by modulating the organization of the actin cytoskeleton mainly through Rho family GTPases. Eph receptors and ephrin ligand signaling regulaes many aspects of cell interaction in the developing neocortex, including migration and axonal target recognition. Ephrin signaling plays an important role in guiding neurons and their projections during embryonic development1,2.In mammals, the Eph receptor tyrosine kinases family has 14 members that are divided into 2 subclasses, the EphA (A1-A8 and A10) and EphB (B1-B4 and B6). This division is based on the sequence similarity of their extracellular domains. EphA receptors bind to glycosylphosphatidyl inositol-anchored ephrin-A ligands, and EphB receptors bind to transmembrane ephrin-B proteins. The ephrin-A proteins include five members ephrin-A1 to ephrin-A5 whereas ephrin-B proteins include three ephrin-B1 to ephrin-B32,3.Ephrin-A2 protein is abundantly expressed in retinal axons. Mutations in Ephrin-A2 disrupts topographic ordering of projections in the developing visual system. A knockout of ephrin-A2 in adult mice shows focal patches of disorganized neocortical laminar architecture, ranging in severity from reduced neuronal density to a complete lack of neurons1,2.

Gene ID: 13637

NCBI Accession: NM_001405

UniProt: P52801