Ephrin A5 antibody (Extracellular)

Catalog No. ABIN7043130

Product Details anti-Ephrin A5 Antibody

Target: Ephrin A5 (EFNA5)

Binding Specificity: AA 172-186, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Ephrin A5

Specificity: Extracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-Ephrin-A5 (extracellular) Antibody (ABIN7043130, ABIN7044259 and ABIN7044260) is a highly specific antibody directed against an epitope of human EFNA5. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize EFNA5 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRVFDVNDKVENSLE, corresponding to amino acid residues 172-186 of human Ephrin-A5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235310)

Blocking Peptide: (ABIN7235310)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Ephrin A5

Target: Ephrin A5 (EFNA5)

Alternative Name: EFNA5 (EFNA5 Products)

Background: EPH-related receptor tyrosine kinase ligand 7, LERK7, AL-1, EFNA5,The Eph family of tyrosine kinase receptor signaling pathway is important for a variety of physiological processes during development including cell morphology, proliferation, migration and axonal guidance, dendritic spine formation and neural plasticity. Eph receptors mediate forward signaling in one cell while ephrin ligands transmit reverse signaling in an adjacent cell1-3.Eph receptors consist of two large subfamilies: EphA and EphB that bind to either glycophosphatidylinositol (GPI)-anchored ephrin-A ligands or transmembrane ephrin-B ligands respectively. Ephrin ligands are divided into ephrin-A (1 to 5) and ephrin-B (1 to 3)1,2.Ephrin-A5 is predominantly expressed in the quiescent anterior lens epithelial cells and is highly expressed in different layers of the cortex2,3.Studies show that mutations in ephrin-A5 are linked to congenital and age-related cataracts in humans and mice. Loss of ephrin-A5 causes abnormal localization of E-cadherin and β-catenin in anterior epithelial cells and epithelial-mesenchymal-transition2.

Alternative names: Ephrin A5, EPH-related receptor tyrosine kinase ligand 7, LERK7, AL-1, EFNA5

Gene ID: 1946

NCBI Accession: NM_001962

UniProt: P52803

Pathways: RTK Signaling