G Protein-Coupled Receptor 119 antibody (2nd Cytoplasmic Loop)

Catalog No. ABIN7886423

The Rabbit Polyclonal anti-G Protein-Coupled Receptor 119 antibody is suitable to detect G Protein-Coupled Receptor 119 in samples from Rat. It has been validated for WB.

Quick Overview for G Protein-Coupled Receptor 119 antibody (2nd Cytoplasmic Loop) (ABIN7886423)

Target: G Protein-Coupled Receptor 119 (GPR119)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G Protein-Coupled Receptor 119 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-G Protein-Coupled Receptor 119 Antibody

Binding Specificity: 2nd Cytoplasmic Loop, AA 186-200

Purpose: A Rabbit Polyclonal Antibody to Glucose-Dependent Insulinotropic Receptor (GPR119)

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: human - 13,15 amino acid residues identical,Mouse - 14

Characteristics: Anti-GPR119 Antibody is directed against an epitope of the rat Glucose-dependent insulinotropic receptor. Anti-GPR119 Antibody (ABIN7043147, ABIN7044419 and ABIN7044420) can be used in western blot analysis. It has been designed to recognize GPR119 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CDMLK IASVHSQHIR, corresponding to amino acid residues 186-200 of rat GPR119

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235360)

Blocking Peptide: (ABIN7235360)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for G Protein-Coupled Receptor 119

Target: G Protein-Coupled Receptor 119 (GPR119)

Alternative Name: GPR119

Background:

Synonyms: Glucose-dependent insulinotropic receptor, GPCR2

Description: Glucose-dependent insulinotropic receptor (GPR119) is a rhodopsin-type member of the G protein-coupled receptor (GPCRs) superfamily. Like all members, it has seven transmembrane spanning domains, an extracellular N-terminus and intracellular C-terminus. Activation of the receptor leads to a rise in intracellular cAMP - suggesting a stimulation of adenylate cyclase, possibly by Gαs coupling. It is expressed mostly in enteroendocrine cells of the gastrointenstinal tract and b-cells of the pancreas. It was stipulated that GPR119 is a close relative to the cannabinoid receptors1,2.Phospholipids and fatty acid amides are endogenous ligands for GPR119. Notably, oleoethanolamide (OEA) seems to be an important agonist. Stimulation of GPR119 by its agonists causes b-cells to release insulin and enteroendocrine cells to release glucagon-like peptide-1 (GLP-1), this attribute makes GPR119 a strong therapeutic candidate for type-2 diabetes and obesity3,4. Furthermore, its ability to release GLP-1 upon stimulation by dietary fat has gained it the title "fat sensor"5 .

Gene ID: 302813

NCBI Accession: NM_178471

UniProt: Q7TQN8

Pathways: Hormone Transport, Peptide Hormone Metabolism