GPR52 antibody (AA 239-253)

Catalog No. ABIN7043161

Product Details anti-GPR52 Antibody

Target: GPR52 (G Protein-Coupled Receptor 52 (GPR52))

Binding Specificity: AA 239-253

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR52 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to GPR52

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 13,Rat - 14,15 amino acid residues identical

Characteristics: Anti-GPR52 Antibody (ABIN7043161, ABIN7044453 and ABIN7044454)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize GPR52 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRRARFPSHEVEASR, corresponding to amino acid residues 239 - 253 of mouse GPR52

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GR058

Blocking Peptide: BLP-GR058

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR52

Target: GPR52 (G Protein-Coupled Receptor 52 (GPR52))

Alternative Name: GPR52 (GPR52 Products)

Background: G-Protein Coupled Receptor 52,G-Protein Coupled Receptor 52 (GPR52) is a conserved class-A orphan receptor, a member of the of the GPCR superfamily. The native ligand for GPR52 is unknown but the receptor has a high level of basal activity not related to ligand binding. Structure analysis shows that GPR52 extracellular loop 2 occupies the orthosteric binding pocket and operates as a built-in agonist, enabling an intrinsically high level of basal activity. The receptor also features a side pocket for ligand binding3.Tissue distribution analysis of human, mouse, and rat indicates that GPR52 is expressed exclusively in the brain, especially in striatum. In the human brain, GPR52 expression profile overlaps with the distribution of D1 and D2 dopamine receptors which are associated with its function1.GPR52 inhibits dopamine D2 receptor signaling and activates dopamine D1/NMDA receptors via intracellular cAMP accumulation2.GPR52 is involved in the manifestation of schizophrenia, Huntington's disease, cognitive impairment and hyperactivity and several other psychiatric disorders and hence it represents a promising therapeutic target. Agonists may potentially act as a novel class of antipsychotics1,2.

Alternative names: G-Protein Coupled Receptor 52, GPR52

Gene ID: 620246

NCBI Accession: NM_005684

UniProt: P0C5J4