GPR52 antibody (3rd Intracellular Loop)

Catalog No. ABIN7043161

This Rabbit Polyclonal antibody specifically detects GPR52 in WB, IHC and IF. It exhibits reactivity toward Mouse.

Quick Overview for GPR52 antibody (3rd Intracellular Loop) (ABIN7043161)

Target: GPR52 (G Protein-Coupled Receptor 52 (GPR52))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR52 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GPR52 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 239-253

Purpose: A Rabbit Polyclonal Antibody to GPR52

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 13,Rat - 14,15 amino acid residues identical

Characteristics: Anti-GPR52 Antibody (ABIN7043161, ABIN7044453 and ABIN7044454) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize GPR52 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DRRARFPSHEVEASR, corresponding to amino acid residues 239 - 253 of mouse GPR52

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235423)

Blocking Peptide: (ABIN7235423)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GPR52

Target: GPR52 (G Protein-Coupled Receptor 52 (GPR52))

Alternative Name: GPR52

Background:

Synonyms: G-Protein Coupled Receptor 52

Description: G-Protein Coupled Receptor 52 (GPR52) is a conserved class-A orphan receptor, a member of the of the GPCR superfamily. The native ligand for GPR52 is unknown but the receptor has a high level of basal activity not related to ligand binding. Structure analysis shows that GPR52 extracellular loop 2 occupies the orthosteric binding pocket and operates as a built-in agonist, enabling an intrinsically high level of basal activity. The receptor also features a side pocket for ligand binding3.Tissue distribution analysis of human, mouse, and rat indicates that GPR52 is expressed exclusively in the brain, especially in striatum. In the human brain, GPR52 expression profile overlaps with the distribution of D1 and D2 dopamine receptors which are associated with its function1.GPR52 inhibits dopamine D2 receptor signaling and activates dopamine D1/NMDA receptors via intracellular cAMP accumulation2.GPR52 is involved in the manifestation of schizophrenia, Huntington's disease, cognitive impairment and hyperactivity and several other psychiatric disorders and hence it represents a promising therapeutic target. Agonists may potentially act as a novel class of antipsychotics1,2.

Gene ID: 620246

NCBI Accession: NM_005684

UniProt: P0C5J4