SLC6A1 antibody (2nd Extracellular Loop)

Catalog No. ABIN7043178

The Rabbit Polyclonal anti-SLC6A1 antibody (ABIN7043178) specifically detects SLC6A1 in WB, IHC, IF, FACS and LCI. The antibody is reactive with Rat samples.

Quick Overview for SLC6A1 antibody (2nd Extracellular Loop) (ABIN7043178)

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-SLC6A1 Antibody

Binding Specificity: 2nd Extracellular Loop, AA 194-206

Purpose: A Rabbit Polyclonal Antibody to GABA Transporter 1 (GAT-1)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-GABA Transporter 1 (extracellular) Antibody (ABIN7043178, ABIN7044459 and ABIN7044460) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ERNMHQMTDGLDK, corresponding to amino acids residues 194-206 of rat GAT-1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235465)

Blocking Peptide: (ABIN7235465)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SLC6A1

Target: SLC6A1 (GAT1) (GABA Transporter 1 (GAT1))

Alternative Name: SLC6A1

Background:

Synonyms: GABATR, GABT1, GABATHG, SLC6A1, Na+/Cl-- dependent GABA transporter 1

Description: γ-Aminobutiric acid (GABA) is the major inhibitory neurotransmitter in the mammalian central nervous system. Transmission via GABA can be halted by its reuptake through GABA transporters (GATs). These membrane proteins belong to the super family of slc6 genes which encode transporters responsible for the uptake of dopamine, serotonin, norepinephrine, glycine and GABA in a Na+-dependent manner1,2.To date, four GATs have been identified: GAT-1, GAT-2, GAT-3 and BGT-1. Like all members of the slc6 family, they have twelve transmembrane domains and short intracellular N- and C-termini1. GATs couple the transport of one GABA molecule to 2 Na+ ions and one Cl- ion3. GAT-1 activity can be regulated at the level of its plasma membrane expression which is in part regulated by its trafficking via phosphorylation of residues in intracellular loops1.GAT-1 expression is restricted to the central nervous system, specifically to axon terminals, in the neocortex and in some astrocytic processes4.GAT-1 may be implicated in various psychological and neurological disorders such as schizophrenia, epilepsy and cerebral ischemia1,5-7.

Gene ID: 79212

NCBI Accession: NM_003042

UniProt: P23978