GJA4 antibody (Intracellular)

Catalog No. ABIN7886191

This Rabbit Polyclonal antibody specifically detects GJA4 in WB. It exhibits reactivity toward Rat.

Quick Overview for GJA4 antibody (Intracellular) (ABIN7886191)

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GJA4 Antibody

Binding Specificity: AA 131-144, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Connexin-37

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-Connexin-37 Antibody is directed against an epitope of rat Connexin-37. Anti-Connexin-37 Antibody (ABIN7043200, ABIN7044081 and ABIN7044082) can be used in western blot analysis. It has been designed to recognize Cx37 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37

Isotype: IgG

Application Details

Application Notes:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235535)

Blocking Peptide: (ABIN7235535)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GJA4

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Alternative Name: GJA4

Background:

Synonyms: CX37, Gap junction α4 protein, GJA4

Description: Connexins (Cxs) are a family of transmembrane proteins comprising 21 members in humans that forms gap junction channels (GJCs). Connexins are divided in four groups: α, β, γ and δ. Cx37 is also named α4 since it is the fourth connexin of the alpha group that was identified1,2. Connexins do not function as single proteins, six connexins assemble into connexons (hemi-channels), and two connexons from neighboring cells dock in the extracellular space to form a full gap junction channel. Connexins are responsible for mediating direct communication between adjacent cells by the exchange of ions and small signaling molecules and cytosolic factors1,3. Their structure consists of four transmembrane helices, two extracellular loops, a cytoplasmic loop, a cytoplasmic NH2- and COOH-termini1,2.Connexin-37 is encoded by the GJA4 gene and is expressed in vascular endothelial cells. The protein is responsible for regulating and controlling the growth, proliferation and regeneration of the vascular endothelium by mediating the signal transduction between smooth muscle and endothelial cells. Cx37 knockout mice have a tendency for developing endarterium atheromatous plaques4. Also, a polymorphism in the gene that encodes for Cx37, is associated with increased risk for atherosclerosis and myocardial infarction1.

Gene ID: 25655

NCBI Accession: NM_002060

UniProt: Q03190

Pathways: Cell-Cell Junction Organization