GIPR antibody (Extracellular)

Catalog No. ABIN7043209

Product Details anti-GIPR Antibody

Target: GIPR (Gastric Inhibitory Polypeptide Receptor (GIPR))

Binding Specificity: AA 25-38, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIPR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Mouse - 13, human - 12

Characteristics: Anti-GIPR (extracellular) Antibody (ABIN7043209, ABIN7044412 and ABIN7044413)) is a highly specific antibody directed against an epitope of the rat Gastric inhibitory polypeptide receptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize GIPR from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EGQTTGELYQRWER,corresponding to amino acid residues 25-38 of rat GIPR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GR025

Blocking Peptide: BLP-GR025

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GIPR

Target: GIPR (Gastric Inhibitory Polypeptide Receptor (GIPR))

Alternative Name: GIPR (GIPR Products)

Background: Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor,Gastric inhibitory polypeptide (GIP) is a 42-amino acid hormone released from the gastrointestinal tract and was first found to inhibit the secretion of hydrochloric acid from stomach parietal cells. GIP also stimulates postprandial insulin secretion from pancreatic β-cells following a pathway activated by the binding of GIP to its specific receptor thus making GIP, along with GLP-1, a major potentiator of glucose induced insulin secretion1.The human GIP receptor is a 466 amino-acid G-coupled protein receptor that is 81.5 % and 81.2 % identical to the hamster and rat receptors comprising seven potential transmembrane domains. This property is shared with glucagon and secretin receptor families.GIP has an affinity of Kd~0.2 nM to its receptor. GIPR activates several intracellular pathways including MAP kinase, phospholipase A2 but primarily activates adenylyl cyclase causing an increase in cAMP. GIP has been also found to be expressed outside the gut in organs such as the heart, brain and adipose tissue.In patients with Type 2 Diabetes-Mellitus, GIP levels are normal or at most minimally reduced therefore suggesting that there is a resistance of β-cells to GIP caused by a change in receptor expression signaling or mutation. In addition, mice whose GIPR gene expression is disrupted, following three months of high fat feeding, show reduced weight gain and adipose tissue compared to regular mice suggesting the elimination of this receptor may protect against the onset of severe obesity2.

Alternative names: GIPR, Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor

Gene ID: 25024

NCBI Accession: NM_000164

UniProt: P43219

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling