GRPR antibody (Extracellular)

Catalog No. ABIN7043210

Product Details anti-GRPR Antibody

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Binding Specificity: AA 287-300, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRPR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Bombesin Receptor 2/Gastrin-releasing Peptide Receptor

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,dog - identical

Characteristics: Anti-Bombesin Receptor 2 (GRPR) (extracellular) Antibody is directed against an epitope located in the 3rd extracellular loop of the human BB2 receptor (gastrin-releasing peptide receptor). Anti-Bombesin Receptor 2 (extracellular) (GRPR) Antibody (ABIN7043210 and ABIN7043937)) can be used in western blot analysis, as well as immunocytochemical and immunohistochemical applications, and will recognize BB2R from human, rat, mouse, and dog samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSYHYSEVDTSMLH, corresponding to amino acid residues 287-300 of human BB2R 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Cited Application: IHC

Negative Control: BLP-BR002

Blocking Peptide: BLP-BR002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRPR

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Alternative Name: GRPR (GRPR Products)

Background: BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor,Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP, a 27 amino acid homologue) and neuromedin B (NMB, a 10 amino acid homologue).BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R.1 BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified.All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.2GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes.2 In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation.3Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

Alternative names: Bombesin Receptor 2 (GRPR), BB2R, Gastrin-releasing peptide receptor, GRP-preferring bombesin receptor

Gene ID: 2925

NCBI Accession: NM_005314

UniProt: P30550