GRIK5 antibody (Extracellular)

Catalog No. ABIN7043241

The Rabbit Polyclonal anti-GRIK5 antibody (ABIN7043241) specifically detects GRIK5 in WB, IHC, IF and IC. The antibody is reactive with Rat samples.

Quick Overview for GRIK5 antibody (Extracellular) (ABIN7043241)

Target: GRIK5 (Glutamate Receptor, Ionotropic, Kainate 5 (GRIK5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-GRIK5 Antibody

Binding Specificity: AA 430-442, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Kainate Receptor GluK5

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse - identical

Characteristics: Anti-GRIK5 (GluK5) (extracellular) Antibody is directed against an epitope of the rat kainate receptor GluK5. Anti-GRIK5 (GluK5) (extracellular) Antibody (ABIN7043241, ABIN7044382 and ABIN7044383) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GluK5 from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRPNFQALSGNER,corresponding to amino acid residues 430-442 of rat kainate receptor GluK5

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235669)

Blocking Peptide: (ABIN7235669)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRIK5

Target: GRIK5 (Glutamate Receptor, Ionotropic, Kainate 5 (GRIK5))

Alternative Name: GRIK5

Background: Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2,There are three glutamate receptor superfamilies defined by the specific agonist binding to them - NMDA, AMPA and kainate.Several types of kainate receptors have been cloned and classified. Two of these have a high affinity to kainate (GLUK1 and GLUK2), while GLUK5, as well as other members of the family, have a lower affinity1. GLUK5, GLUK6 and GLUK7 have a 75-80 % identical amino acid sequence making them a separate subfamily from the other kainate receptors.The GLUK5 receptor, similar to its subfamily members, is a ligand-gated ion channel. It has an extracellular N-terminus comprised of eight glycosylation consensus sites, four transmembrane domains arranged in a three-plus-one pattern with a large intracellular domain between units three and four and a C-terminus2. GLUK5 is expressed in DRG neuron cell bodies where currents in these cells are preferentially induced by kainate and not AMPA3 and also in trigeminal neurons where kainate receptor antagonists are effective in a dural plasma protein extravasation model of migraine in rats4.Little is known about the role of GLUK5 in pathologies. A study examining the role of the receptor in brain pathology using GLUK5 antagonists on hippocampal cells slices, found that blocking of the kainate receptor prevents the development of epileptic activity evoked by muscarinic antagonist. The administration of GLUK5 antagonists to conscious mice in the same study showed that the antagonist prevents limbic seizures induced by hippocampal pilocarpine perfusion without evident side effects. These findings suggest that GLUK5 antagonists may be effective in the treatment of epilepsy and further research in this field is required5.

Alternative names: GRIK5 (GluK5), Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2

Gene ID: 24407

NCBI Accession: NM_001301030

UniProt: Q63273

Pathways: Carbohydrate Homeostasis, Synaptic Membrane, Maintenance of Protein Location, Synaptic Vesicle Exocytosis