GRIN2C antibody (Extracellular)

Catalog No. ABIN7043244

The Rabbit Polyclonal anti-GRIN2C antibody (ABIN7043244) specifically detects GRIN2C in WB, IHC, IF and IC. The antibody is reactive with Rat samples.

Quick Overview for GRIN2C antibody (Extracellular) (ABIN7043244)

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-GRIN2C Antibody

Binding Specificity: AA 365-377, Extracellular

Purpose: A Rabbit Polyclonal Antibody to NMDA Receptor 2C (GRIN2C)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 12,13 amino acid residues identical

Characteristics: Anti-NMDAR2C (GRIN2C) (extracellular) Antibody is directed against an epitope of rat NMDA receptor 2C. Anti-NMDAR2C (GRIN2C) (extracellular) Antibody (ABIN7043244, ABIN7044348 and ABIN7044349) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NR2C from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RHRLWEMVGRWDH, corresponding to amino acid residues 365-377 of rat NMDAR2C

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235677)

Blocking Peptide: (ABIN7235677)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRIN2C

Target: GRIN2C (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2c (GRIN2C))

Alternative Name: GRIN2C

Background: NMDA receptor 2C, NR2C, GluN2C, Ionotropic glutamate receptor subunit ε3, N-methyl-D-aspartate receptor subunit 2C,The NMDA receptors (NMDARs) are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.NMDA are generally highly expressed in the central nervous system, particularly in the brain. In addition, NMDAR2C has been also detected in the heart, sketeletal muscle, and pancreas.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.The expression and function of NMDA receptors are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Alternative names: NMDAR2C (GRIN2C), NMDA receptor 2C, NR2C, GluN2C, Ionotropic glutamate receptor subunit epsilon3, N-methyl-D-aspartate receptor subunit 2C

Gene ID: 24411

NCBI Accession: NM_000835

UniProt: Q00961

Pathways: Synaptic Membrane