Metabotropic Glutamate Receptor 1 antibody (Extracellular) (Atto 488)

Catalog No. ABIN7043246

The Rabbit Polyclonal anti-Metabotropic Glutamate Receptor 1 antibody is suitable to detect Metabotropic Glutamate Receptor 1 in samples from Rat. It has been validated for IHC and IF.

Quick Overview for Metabotropic Glutamate Receptor 1 antibody (Extracellular) (Atto 488) (ABIN7043246)

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Metabotropic Glutamate Receptor 1 Antibody (Atto 488)

Binding Specificity: AA 501-516, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 1 Conjugated to the Fluorescent Dye ATTO-488

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human,dog - identical

Characteristics: Anti-mGluR1 (extracellular) Antibody (ABIN7043245, ABIN7044330 and ABIN7044331) is a highly specific antibody directed against the extracellular N-terminus domain of rat mGluR1. The antibody can be used in western blot and immunocytochemistry applications, and recognizes mGluR1 in rat, mouse and human samples. \nAnti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody (ABIN7043246) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-mGluR1 (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HEGVLNIDDYKIQMNK, corresponding to amino acids 501-516 of rat mGluR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:20-1:60

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582041)

Blocking Peptide: (ABIN7235681)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Alternative Name: GRM1

Background: Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13,L-Glutamate is the major excitatory neurotransmitter in the central nervous system. It operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group I of the metabotropic glutamate receptors includes the mGluR1 and mGluR5 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus and an intracellular C-terminus. The N-terminus domain of Group I receptors contains the glutamate binding site while the cytoplasmic C-terminus domain has an important role in the regulation of receptor activity through interactions with other proteins such as the Homer adaptor proteins.mGluR1 and mGluR5 receptors signal through Gq/G11, that activates phospholipase C and ultimately produces an increase in inositol trisphosphate and cytosolic Ca2+. Additional downstream signaling pathways include the activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G-proteins have also been reported.mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus.The mGluR1 receptor is involved in several physiological processes such as neuronal development, induction of long-term potentiation (LTP) and depression (LTD) as well as in pathological disorders such as brain trauma, chronic pain, Parkinson's and Huntington's disease.

Alternative names: mGluR1, Metabotropic glutamate receptor 1, GRM1, mGlu1, GPRC1a, PPP1R85, SCAR13

Gene ID: 24414

NCBI Accession: NM_000838

UniProt: P23385