GRM2 antibody (Extracellular)

Catalog No. ABIN7043247

Product Details anti-GRM2 Antibody

Target: GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))

Binding Specificity: AA 109-121, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human,dog,monkey - identical

Characteristics: Anti-mGluR2 (extracellular) Antibody is directed against an epitope of rat mGluR2. The epitope is specific for mGluR2 and will not cross-react with the closely related mGluR3 channel. Anti-mGluR2 (extracellular) Antibody (ABIN7043247, ABIN7044340 and ABIN7044341)) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize mGluR2 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: SLSRGADGSRHIC, corresponding to amino acids 109-121 of rat mGluR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Cited Application: IFC

Negative Control: BLP-GC011

Blocking Peptide: BLP-GC011

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRM2

Target: GRM2 (Glutamate Receptor, Metabotropic 2 (GRM2))

Alternative Name: GRM2 (GRM2 Products)

Background: Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B,L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The metabotropic glutamate receptor family includes eight members (mGluR1-8) that have been divided into three groups based on their sequence homology, pharmacology and signal transduction.Group II of the metabotropic glutamate receptors includes the mGluR2 and mGluR3 receptors. The receptors present the typical G-protein coupled receptor (GPCR) signature topology: seven transmembrane domains with a large extracellular N-terminus domain that contains the glutamate binding site, and an intracellular C-terminus.1,2 mGluR2 and mGluR3 are coupled to Gi/Go and hence inhibit cAMP formation following receptor activation.1,2mGluR2 is widely distributed throughout the brain with high expression in several limbic areas including the cortex, hippocampus and amygdala. mGluR2 is localized primarily presynaptically, although postsynaptic localization has also been described.In line with its presynaptic localization, mGluR2 is thought to function as an autoreceptor in a negative feedback mechanism that suppress further release of glutamate from the cell on which it is expressed.The involvement of mGluR2 in neuronal excitability and synaptic transmission suggests that modulation of this receptor is a promising strategy for the treatment of neurological and neuropsychiatric disorders such as anxiety, schizophrenia, and pain.3

Alternative names: mGluR2, Metabotropic glutamate receptor 2, GRM2, mGlu2, GPRC1B

Gene ID: 24415

NCBI Accession: NM_000839

UniProt: P31421

Pathways: cAMP Metabolic Process, Dicarboxylic Acid Transport