Metabotropic Glutamate Receptor 5 antibody (Extracellular, N-Term) (Atto 594)

Catalog No. ABIN7043251

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody (Atto 594)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Binding Specificity: AA 367-380, Extracellular, N-Term

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Characteristics: Anti-mGluR5 (extracellular) Antibody (ABIN7043250, ABIN7044332 and ABIN7044333)) is a highly specific antibody directed against an extracelluar epitope of rat mGluR5. The antibody can be used in western blot analysis, immunhistochemical and live cell imaging applications. It has been designed to recognize mGluR5 from rat, mouse and human samples. \nAnti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody (#ABIN7043251) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-mGluR5 (extracellular)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: mGluR5 (GRM5 Products)

Background: Alternative names: mGluR5, Metabotropic glutamate receptor 5, GRM5, mGlu5, GPRC1E

Gene ID: 24418

NCBI Accession: NM_000842

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity