Metabotropic Glutamate Receptor 6 antibody (Extracellular)

Catalog No. ABIN7043254

This Rabbit Polyclonal antibody specifically detects Metabotropic Glutamate Receptor 6 in WB. It exhibits reactivity toward Rat.

Quick Overview for Metabotropic Glutamate Receptor 6 antibody (Extracellular) (ABIN7043254)

Target: Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Metabotropic Glutamate Receptor 6 Antibody

Binding Specificity: AA 368-381, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 6

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Mouse, human - 12,rat - 13

Characteristics: Anti-mGluR6 (extracellular) Antibody (ABIN7043254, ABIN7044361 and ABIN7044362) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize mGluR6 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence:  (C)GQSDDSTRK(S)TGEE, corresponding to amino acid residues 368-381 of rat mGluR6

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7235701)

Blocking Peptide: (ABIN7235701)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Metabotropic Glutamate Receptor 6

Target: Metabotropic Glutamate Receptor 6 (GRM6) (Glutamate Receptor, Metabotropic 6 (GRM6))

Alternative Name: GRM6

Background: Metabotropic glutamate receptor 6, GRM6, mGlu6, Gprc1f,Glutamate is the principal excitatory neurotransmitter in the central nervous system. It operates through two main classes of receptors: ionotropic receptors (iGluRs), which are ligand-gated ion channels, and metabotropic receptors (mGluRs), which couple to G-proteins to modulate cellular responses via ion channels or intracellular second messengers.The metabotropic receptors include eight family members (mGluR1-mGluR8) classified into three groups according to their sequence similarities, signal transduction, and agonist selectivity. They all share a common structure, being composed of a single polypeptide with large N-terminal extracellular domain connected through a seven transmembrane domain motif to the intracellular C-terminal tail1,2. The different mGluR subtypes are localized at both presynaptic and postsynaptic membranes, and are involved in the generation of slow excitatory and inhibitory synaptic potentials, modulation of synaptic transmission, synaptic integration, and plasticity3.mGluR6 belongs to the third group of metabotrophic glutamate receptors. Its expression in the central nervous system is restricted to retinal rod bipolar cells, and it's localized to their postsynaptic dendritic membranes3,4. In the peripheral nervous system, it is also detected in bone marrow stromal cells6. mGluR6 inhibits adenylate cyclase, and was shown to inhibit Ca2+ influx and nitric oxide synthase activity3,5,6.

Alternative names: mGluR6, Metabotropic glutamate receptor 6, GRM6, mGlu6, Gprc1f

Gene ID: 24419

NCBI Accession: NM_000843

UniProt: P35349