GRM7 antibody (Extracellular)

Catalog No. ABIN7043255

Product Details anti-GRM7 Antibody

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Binding Specificity: AA 377-390, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC), Immunofluorescence (Cultured Cells) (IF (cc))

Purpose: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 7

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-mGluR7 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR7. Anti-mGluR7 (extracellular) Antibody (ABIN7043255, ABIN7044346 and ABIN7044347)) can be used in western blot, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize mGluR7 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: TISGSKKEDTDRKC, corresponding to amino acid residues 377-390 of rat mGluR7

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GC017

Blocking Peptide: BLP-GC017

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRM7

Target: GRM7 (Glutamate Receptor, Metabotropic 7 (GRM7))

Alternative Name: GRM7 (GRM7 Products)

Background: Metabotropic glutamate receptor 7, GRM7, mGlu7, Gprc1g,Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1.The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2. Indeed, mGluR7 can be detected as five different splice variants3.Like all group III metabotropic glutamate receptors, mGluR7 is localized presynaptically. It is highly expressed in the central nervous system and could also be detected in some peripheral organs like hair cells and spiral ganglion cells of the inner ear3.As opposed to other group III metabotropic glutamate receptors, mGluR7 has very low affinity for its substrate L-glutamate, therefore, only under high synaptic activity is the receptor active. This enables the receptor to act as a negative feedback loop by inhibiting the release of L-glutamate3,4.mGluR7 may play a molecular role in the pathways leading to emotion and cognition4.

Alternative names: mGluR7, Metabotropic glutamate receptor 7, GRM7, mGlu7, Gprc1g

Gene ID: 81672

NCBI Accession: NM_000844

UniProt: P35400

Pathways: Sensory Perception of Sound, cAMP Metabolic Process, Feeding Behaviour