GLRA3 antibody (2nd Cytoplasmic Loop)

Catalog No. ABIN7886410

The Rabbit Polyclonal anti-GLRA3 antibody is suitable to detect GLRA3 in samples from Rat. It has been validated for WB and IHC.

Quick Overview for GLRA3 antibody (2nd Cytoplasmic Loop) (ABIN7886410)

Target: GLRA3 (GLRa3) (Glycine Receptor, alpha 3 (GLRa3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLRA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GLRA3 Antibody

Binding Specificity: 2nd Cytoplasmic Loop, AA 355-368

Purpose: A Rabbit Polyclonal Antibody to Glycine Receptor α3

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Glycine Receptor alpha3 Antibody is directed against an epitope of the rat glycine receptor α3. Anti-Glycine Receptor α3 Antibody (ABIN7043258 and ABIN7044396) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GlyRα3 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRalpha3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7235717)

Blocking Peptide: (ABIN7235717)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GLRA3

Target: GLRA3 (GLRa3) (Glycine Receptor, alpha 3 (GLRa3))

Alternative Name: GLRA3

Background:

Synonyms: Glycine receptor subunit alpha-3, GLRA3, GlyRα3

Description: Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1-α4) encoded by different genes.2-4 In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L and α3K, where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3

Gene ID: 114516

NCBI Accession: NM_001042543

UniProt: P24524