GHSR antibody (Extracellular)

Catalog No. ABIN7043264

The Rabbit Polyclonal anti-GHSR antibody is suitable to detect GHSR in samples from Rat. It has been validated for WB, IHC, FACS, IF, IC and LCI.

Quick Overview for GHSR antibody (Extracellular) (ABIN7043264)

Target: GHSR (Growth Hormone Secretagogue Receptor (GHSR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GHSR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: KO Validated

Product Details anti-GHSR Antibody

Binding Specificity: AA 197-207, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Ghrelin Receptor (GHSR)

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 10,11 amino acid residues identical

Characteristics: Anti-Ghrelin Receptor (GHSR) (extracellular) Antibody (ABIN7043264, ABIN7044417 and ABIN7044418) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize GHSR from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRATEFAVRSG, corresponding to amino acid residues 197-207 of rat Ghrelin receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:800

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235739)

Blocking Peptide: (ABIN7235739)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GHSR

Target: GHSR (Growth Hormone Secretagogue Receptor (GHSR))

Alternative Name: GHSR

Background: Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP,Ghrelin is a 28 amino acid peptide, acylated on a serine residue which is essential for its biological activity1,2. In rodents, an additional form of the peptide could be purified. The peptide is released from the stomach and exerts its effect on the ghrelin receptor (GHS-R). GHS-R is a member of the G-protein coupled receptor (GPCR) superfamily. Like all members, the receptor has seven transmembrane spanning domains and upon activation it stimulates phospholipase C, thereby increasing IP3 and intracellular Ca2+ levels1,3.GHS-R is expressed in the hypothalamus, pituitary and hippocampus1,3.Some of the actions of ghrelin include the stimulation of growth hormone release, food intake, fat accumulation, regulation of memory and learning, activation of reward-related pathways and neuroprotection to name a few1,3.GHS-R 1b is a truncated form of the full length receptor which is also expressed. Although its exact functions are unknown, it is believed that it may be involved in downregulating signaling from the full length receptor4. In addition, it is overexpressed in lung cancer where it dimerizes with neurotensin receptor4,5.

Alternative names: Ghrelin Receptor (GHSR), Growth hormone secretagogue receptor type 1, GHS-R, GH-releasing peptide receptor, GHRP

Gene ID: 84022

NCBI Accession: NM_004122

UniProt: O08725

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Feeding Behaviour