IQGAP1 antibody (Intracellular)

Catalog No. ABIN7043292

Product Details anti-IQGAP1 Antibody

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Binding Specificity: AA 793-808, Intracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IQGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to IQGAP1

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 15,16 amino acid residues identical

Characteristics: Anti-IQGAP1 Antibody (ABIN7043292, ABIN7045192 and ABIN7045193) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IQGAP1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KAYQDRLAYLRSHKDE, corresponding to amino acid residues 793 - 808 of human IQGAP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235826)

Blocking Peptide: (ABIN7235826)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for IQGAP1

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Alternative Name: IQGAP1 (IQGAP1 Products)

Background: IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195,IQGAP1 is a 190 kDa (1657 amino acid) protein that is ubiquitously expressed and contains six distinct protein-interacting domains with six coiled coil regions1.The Ras GTPase-activating-like protein is a scaffold protein involved in regulating various cellular processes, from organizing the actin cytoskeleton, transcription, cell adhesion, and regulating the cell cycle2,3.IQGAP1 binds to activated CDC42 but does not stimulate its GTPase activity and it may have a role in cell cycle progression following DNA damage1,2. The protein also regulates NR2A NMDA receptor signaling where it influences cognitive processes4. It also regulates MAPK and Wnt/β-catenin signaling pathways5. It is thought to contribute to tumorigenesis by stimulating cell motility and invasion6.

Alternative names: IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195

Gene ID: 8826

NCBI Accession: NM_003870

UniProt: P46940

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2