KISS1R antibody (Extracellular)

Catalog No. ABIN7043294

The Rabbit Polyclonal anti-KISS1R antibody has been validated for WB, IHC, IF, FACS and LCI. It is suitable to detect KISS1R in samples from Human.

Quick Overview for KISS1R antibody (Extracellular) (ABIN7043294)

Target: KISS1R (KISS1 Receptor (KISS1R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KISS1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-KISS1R Antibody

Binding Specificity: AA 292-305, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Kisspeptin Receptor

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Rat

Predicted Reactivity: mouse - 12,14 amino acid residues identical,Rat - 13

Characteristics: Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (ABIN7043294, ABIN7044548 and ABIN7044549) is a highly specific antibody directed against an epitope of the human kisspeptin receptor. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KISS1R from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:500

Comment:

Cited Application: IHC|ICC

Negative Control: (ABIN7235833)

Blocking Peptide: (ABIN7235833)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KISS1R

Target: KISS1R (KISS1 Receptor (KISS1R))

Alternative Name: KISS1R

Background: Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12,The kisspeptin receptor (KISS1R, GPCR54) is a member of the G-protein coupled receptor superfamily. KISS1R natural ligands are peptides called kisspeptins which are encoded by the Kiss1 gene. The Kiss1 gene was originally discovered by Hershey scientists who named it after their famous chocolate, the "Kiss".The kisspeptins, acting through KISS1R, were found to be potent inhibitors of metastasis in a variety of cancer cell types including thyroid and pancreatic cancer cells. KISS1R and kisspeptins are involved in the direct regulation of the reproductive neuroendocrine axis.KISS1R is widely distributed throughout the brain, where it is most highly expressed in the hypothalamic and amygdaloid nuclei. High expression was also found in the pancreas and placenta. Lower-level expression was found in a variety of other peripheral tissues including peripheral blood leukocytes, kidney, lung, testes, spleen, thymus, adrenal glands and lymph nodes.1-3

Alternative names: KISS1 Receptor (GPR54), Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12

Gene ID: 84634

NCBI Accession: NM_032551

UniProt: Q969F8