LINGO1 antibody (Extracellular)

Catalog No. ABIN7043298

Product Details anti-LINGO1 Antibody

Target: LINGO1 (Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1))

Binding Specificity: AA 507-521, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LINGO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to LINGO-1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti- (extracellular) Antibody is directed against an extracellular epitope of mouse . Anti- (extracellular) Antibody (ABIN7043298, ABIN7044790 and ABIN7044791) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HVRSYSPDWPHQPNK, corresponding to amino acid residues 507-521 of mouse LINGO-1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Negative Control: (ABIN7235848)

Blocking Peptide: (ABIN7235848)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LINGO1

Target: LINGO1 (Leucine Rich Repeat and Ig Domain Containing 1 (LINGO1))

Alternative Name: LINGO1 (LINGO1 Products)

Background: Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat neuronal protein 6A, LRRN6A, LERN1,LINGO-1 (LRR and Ig domain-containing, nogo receptor interacting protein) is a protein highly expressed in the brain but not in other tissues. There are currently two other known human paralogs- LINGO-2 and LINGO-3. LINGO-1 contains 12 leucine-rich repeat (LRR) motifs flanked by N- and C- terminal capping domains, one immunoglobulin (Ig) domain, a transmembrane domain and a short cytoplasmic tail. The cytoplasmic tail contains a canonical epidermal growth factor receptor-like tyrosine phosphorylation site. LINGO-1 is highly conserved evolutionary with human and mouse orthologues, sharing 99.5 % identity. LINGO-1 binds both to Nogo-66 receptor (NgR1) and to p75 neurotrophin receptor simultaneously and can form a ternary complex1.In animal models, LINGO-1 expression is upregulated in rat spinal cord injury, experimental autoimmune encephalomyelitis, neurotoxic lesions and glaucoma. In humans, LINGO-1 expression is increased in oligodendrocyte progenitor cells from demyelinated white matter of multiple sclerosis post-mortem samples, and in dopaminergic neurons from Parkinson's disease brains. LINGO-1 negatively regulates oligodendrocyte differentiation and myelination, neuronal survival and axonal regeneration by activating the RhoA and inhibiting protein kinase B (Akt) phosphorylation signaling pathways2.

Alternative names: LINGO-1, Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat neuronal protein 6A, LRRN6A, LERN1

Gene ID: 235402

NCBI Accession: NM_032808

UniProt: Q9D1T0