LRRC26 antibody (Intracellular)

Catalog No. ABIN7043299

Product Details anti-LRRC26 Antibody

Target: LRRC26 (Leucine Rich Repeat Containing 26 (LRRC26))

Binding Specificity: AA 301-314, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRC26 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to LRRC26

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Won't recognize human LRRC26.

Predicted Reactivity: Mouse - 12,14 amino acid residues identical

Characteristics: Anti-LRRC26 Antibody (ABIN7043299, ABIN7044982 and ABIN7044983)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LRRC26 from rat and mouse samples. It does not recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRTTVRHLIRRQPD, corresponding to amino acid residues 301-314 of rat LRRC26

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-PC070

Blocking Peptide: BLP-PC070

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for LRRC26

Target: LRRC26 (Leucine Rich Repeat Containing 26 (LRRC26))

Alternative Name: LRRC26 (LRRC26 Products)

Background: Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC,BK (KCa1.1) channels are critically involved in diverse physiological processes in both electrically excitable and non-excitable cells.Leucine-rich repeat containing protein 26 (also known as BK channel auxiliary gamma subunit or LRRC26) is a single spanning transmembrane protein of ~37 kDa. LRRC26 protein contains five major leucine rich repeats in its extracellular domain which are all required for modulating BK channel activity. The single transmembrane segment of LRRC26 is required for co-assembly with the BKα subunit complex.When LRRC26 is acutely knocked down in LNCaP cells, the voltage-dependent activation of endogenous BK current shifts from a low to a high V1/2 value, whereas recombinant expression of LRRC26 cDNA in naive cells (such as PC3 prostate cancer cells and HEK 293 cells) normally lacking LRRC26 shifts BK channel activation from high to low V1/2 values, reproducing the channel behavior observed in LNCaP cells. This findings indicate that LRRC26 is sufficient to impart low voltage gating behavior to wild-type pore-forming, BKα subunits.LRRC26 lowers the energy barrier for activation of BK channels, and is able to stabilize channel opening in response to voltage, thereby maintaining channels in the open state to a much greater extent at any given membrane potential1.LRRC26 is expressed in cerebral artery myocytes where it is primarily plasma membrane localized and associated with BKα subunits. LRRC26 serves as an arterial myocyte BK channel auxiliary γ subunit that elevates voltage and apparent Ca2+ sensitivity to induce vasodilation2.

Alternative names: LRRC26, Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC

Gene ID: 311803

NCBI Accession: NM_001013653

UniProt: Q6P7C4