MCHR1 antibody (Extracellular)

Catalog No. ABIN7043320

Product Details anti-MCHR1 Antibody

Target: MCHR1 (Melanin-Concentrating Hormone Receptor 1 (MCHR1))

Binding Specificity: AA 7-20, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCHR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 13,Mouse - 16,17 amino acid residues identical

Characteristics: Anti-MCHR1 (extracellular) Antibody (ABIN7043320 and ABIN7044603)) is a highly specific antibody directed against an epitope of the rat Melanin-concentrating hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize MCHR1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CMDLQTSLLSTGPNASN, corresponding to amino acid residues 7-20 of rat MCHR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-MR041

Blocking Peptide: BLP-MR041

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MCHR1

Target: MCHR1 (Melanin-Concentrating Hormone Receptor 1 (MCHR1))

Alternative Name: MCHR1 (MCHR1 Products)

Background: Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1,Melanin-concentrating hormone (MCH) is a 19 amino acid peptide originally isolated from the salmon pituitary gland (in fish the length of the peptide is 17 amino acids)1. In rodents and humans the peptide is synthesized in the brain and is involved in a number of physiological effects, although it is best known for its central role in the control of appetite control. For example, central administration of MCH in mice leads to obesity and conversely, knockout of the MCH gene in mice yields lean animals with an increased metabolic rate and reduced body fat2,3.MCH exerts its effects by binding MCHR1 and MCHR2, two receptors belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members of the family, MCHR1 and MCHR2 have seven transmembrane domains, an extracellular N-terminal and intracellular C-terminal tails. MCHR1 and MCHR2 activation both lead to an increase in intracellular Ca2+ concentration3.In rat, MCHR1 expression is quite broad, and is strongly detected in the brain. Expression of the receptor is also seen in the eye, skeletal muscle, tongue, pituitary, intestine and lymphocytes4,5. MCHR1 is also expressed in adipose tissue where it regulates leptin secretion3. In human, MCHR1 is mostly localized to the brain and pituitary. MCHR2 displays similar localization to that of MCHR1 in the brain5,6. As MCHR2 is a pseudogene in rodents, studying its function is quite challenging. In the brain, MCHR1 is localized in many areas that are known to be involved in various psychological disorders. Indeed, MCHR1-/- animals display antianxiety- and antidepressant-like behavior7,8. Therefore, antagonizing the activity of MCHR1 could indeed serve as a means to treat depression and obesity9.

Alternative names: MCHR1, Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1

Gene ID: 83567

NCBI Accession: NM_005297

UniProt: P97639

Pathways: Feeding Behaviour