CD56 antibody (Extracellular)

Catalog No. ABIN7043350

This Rabbit Polyclonal antibody specifically detects CD56 in FACS, WB, IHC, IF, IC and LCI. It exhibits reactivity toward Rat.

Quick Overview for CD56 antibody (Extracellular) (ABIN7043350)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: KO Validated

Product Details anti-CD56 Antibody

Binding Specificity: AA 261-276, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 1 (CD56)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,15 amino acid residues identical

Characteristics: Anti-CD56/NCAM1 (extracellular) Antibody (ABIN7043350, ABIN7044686 and ABIN7044687) is a highly specific antibody directed against rat Neural cell adhesion molecule 1. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NCAM1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: (ABIN7236018)

Blocking Peptide: (ABIN7236018)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: NCAM1

Background: Neural cell adhesion molecule 1, NCAM-1,Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3. NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4.Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.

Alternative names: CD56 (NCAM1), Neural cell adhesion molecule 1, NCAM-1

Gene ID: 24586

NCBI Accession: NM_000615

UniProt: P13596