Neuroligin 1 antibody (Extracellular)

Catalog No. ABIN7043361

Product Details anti-Neuroligin 1 Antibody

Target: Neuroligin 1 (NLGN1)

Binding Specificity: AA 648-661, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuroligin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Neuroligin 1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-Neuroligin 1 (extracellular) Antibody is directed against an extracellular epitope of rat Neuroligin 1. Anti-Neuroligin 1 (extracellular) Antibody (ABIN7043361, ABIN7044680 and ABIN7044681)) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NLGN1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat NLGN1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:800

Comment:

Negative Control: BLP-NR035

Blocking Peptide: BLP-NR035

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neuroligin 1

Target: Neuroligin 1 (NLGN1)

Alternative Name: NLGN1 (NLGN1 Products)

Background: NLGN1, NL1,The NLGN1 gene encodes a member of a family of neuronal cell surface proteins. Neuroligins act as ligands for β-neurexins, cell adhesion proteins located presynaptically. Neuroligin and β-neurexin "shake hands," resulting in the connection between the two neurons and the production of a synapse. Neuroligin 1 may be involved in the formation and remodeling of central nervous system synapses1.Proteins of this family may also mediate signaling by recruiting and stabilizing key synaptic components. This is done by interacting with other postsynaptic proteins to localize neurotransmitter receptors and channels in the postsynaptic density as the cell matures1.Mutations in the neuroligin genes are implicated in autism and other cognitive disorders2.Neuroligins have been found to play a role in angiogenesis, specifically in human pheripheral tissues3.Neuroligins bind with the help of Ca2+ to the α-neurexin LNS (laminin, neurexin and sex hormone-binding globulin-like folding units) domains and to the β-neurexin LNS domain which then establishes a heterophilic trans-synaptic recognition code4. This binding results in a heterotetramer made up of a neuroligin-1 dimer and two neurexin-1 β monomers5.

Alternative names: Neuroligin 1, NLGN1, NL1

Gene ID: 116647

NCBI Accession: NM_014932

UniProt: Q62765

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis