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Neuroligin 1 antibody (Extracellular)

NLGN1 Reactivity: Rat WB, IHC, IF, LCI, IC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043361
  • Target See all Neuroligin 1 (NLGN1) Antibodies
    Neuroligin 1 (NLGN1)
    Binding Specificity
    • 18
    • 16
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 648-661, Extracellular
    Reactivity
    • 40
    • 37
    • 23
    • 1
    Rat
    Host
    • 35
    • 26
    • 1
    Rabbit
    Clonality
    • 39
    • 23
    Polyclonal
    Conjugate
    • 21
    • 5
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Neuroligin 1 antibody is un-conjugated
    Application
    • 50
    • 23
    • 22
    • 14
    • 12
    • 11
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)
    Purpose
    A Rabbit Polyclonal Antibody to Neuroligin 1
    Specificity
    Extracellular, N-terminus
    Cross-Reactivity
    Mouse, Rat
    Predicted Reactivity
    Mouse - identical, human - 13,14 amino acid residues identical
    Characteristics
    Anti-Neuroligin 1 (extracellular) Antibody is directed against an extracellular epitope of rat Neuroligin 1. Anti-Neuroligin 1 (extracellular) Antibody (ABIN7043361, ABIN7044680 and ABIN7044681)) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NLGN1 from rat, mouse and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat NLGN1

    Isotype
    IgG
    Top Product
    Discover our top product NLGN1 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

    Application Dilutions Western blot wb: 1:800

    Comment

    Negative Control: BLP-NR035

    Blocking Peptide: BLP-NR035

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    Neuroligin 1 (NLGN1)
    Alternative Name
    NLGN1 (NLGN1 Products)
    Background
    NLGN1, NL1,The NLGN1 gene encodes a member of a family of neuronal cell surface proteins. Neuroligins act as ligands for β-neurexins, cell adhesion proteins located presynaptically. Neuroligin and β-neurexin "shake hands," resulting in the connection between the two neurons and the production of a synapse. Neuroligin 1 may be involved in the formation and remodeling of central nervous system synapses1.Proteins of this family may also mediate signaling by recruiting and stabilizing key synaptic components. This is done by interacting with other postsynaptic proteins to localize neurotransmitter receptors and channels in the postsynaptic density as the cell matures1.Mutations in the neuroligin genes are implicated in autism and other cognitive disorders2.Neuroligins have been found to play a role in angiogenesis, specifically in human pheripheral tissues3.Neuroligins bind with the help of Ca2+ to the α-neurexin LNS (laminin, neurexin and sex hormone-binding globulin-like folding units) domains and to the β-neurexin LNS domain which then establishes a heterophilic trans-synaptic recognition code4. This binding results in a heterotetramer made up of a neuroligin-1 dimer and two neurexin-1 β monomers5.

    Alternative names: Neuroligin 1, NLGN1, NL1
    Gene ID
    116647
    NCBI Accession
    NM_014932
    UniProt
    Q62765
    Pathways
    Synaptic Membrane, Synaptic Vesicle Exocytosis
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