Neuroligin 3 antibody (Extracellular, N-Term)

Catalog No. ABIN7886634

The Rabbit Polyclonal anti-Neuroligin 3 antibody is suitable to detect Neuroligin 3 in samples from Rat. It has been validated for WB and IHC.

Quick Overview for Neuroligin 3 antibody (Extracellular, N-Term) (ABIN7886634)

Target: Neuroligin 3 (NLGN3)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuroligin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Neuroligin 3 Antibody

Binding Specificity: AA 656-671, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Neuroligin 3

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Neuroligin 3 (extracellular) Antibody (ABIN7043363, ABIN7044684 and ABIN7044685) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize NLGN3 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SHITRRPNGKTWSTKR, corresponding to amino acid residues 656-671 of rat NLGN3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236056)

Blocking Peptide: (ABIN7236056)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Neuroligin 3

Target: Neuroligin 3 (NLGN3)

Alternative Name: NLGN3

Background:

Synonyms: NLGN3, Gliotactin homolog, HNL3, NL3

Description: Neuroligins (Nlgns) are a family of postsynaptic transmembrane cell adhesion proteins that play a crucial role in excitation and inhibition balance through their regulation of GABAergic and glutamatergic signaling. 5 Neuroligin family members have been identified in humans: NL1, NL2, NL3, NL4X, and NL4Y. NL 1-3 have close homologs in mice. Neurogilins have been identified in rodents, chicken, Drosophila, and C. elegans. Neuroligin structure contains extracellular acetylcholinesterase like domain that lacks esterase activity, a single transmembrane domain, and a short cytoplasmic tail containing a type I PDZ-domain-binding motif that functions in intracellular protein-protein interactions and signaling processes. Neuroligins and neurexins are known to interact with each other1,2.Neuroligin 3 is encoded by the Nlgn3 gene and seems to be ubiquitously expressed in the brain. Despite this fact, Neuroligin 3 functions in synaptic transmission appears to be region specific, affecting the somatosensory cortex and the hippocampus differently2.In humans, a missense mutation in Nlgn3 (Nlgn3R451C) causing reduced synaptic expression of the protein is associated with non-syndromic autism spectrum disorders. In addition, Nlgn3 deletion shows impaired social interaction and vocal communication in rodent models.

Gene ID: 171297

NCBI Accession: NM_018977

UniProt: Q62889

Pathways: Synaptic Membrane