NMBR antibody (AA 241-256)

Catalog No. ABIN7043364

Product Details anti-NMBR Antibody

Target: NMBR (Neuromedin B Receptor (NMBR))

Binding Specificity: AA 241-256

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMBR antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Bombesin Receptor 1/Neuromedin B Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,dog - identical, rat - 15,16 amino acid residues identical

Characteristics: Anti-Bombesin Receptor 1 (NMBR) Antibody is directed against a well conserved epitope located in the 3rd intracellular loop of the human BB1 receptor (neuromedin B receptor). Anti-Bombesin Receptor 1 (NMBR) Antibody (ABIN7043364 and ABIN7043939)) can be used in western blot and immunocytochemical applications, and will recognize BB1R from human, rat, mouse, and dog samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KSAHNLPGEYNEHTKK, corresponding to amino acid residues 241-256 of human BB1R 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:1000

Comment:

Negative Control: BLP-BR004

Blocking Peptide: BLP-BR004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NMBR

Target: NMBR (Neuromedin B Receptor (NMBR))

Alternative Name: NMBR (NMBR Products)

Background: BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor,Bombesin receptor 1 (BB1) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was initially isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP) and neuromedin B (NMB), 27 and 10 amino acid homologues, respectively.1BB1 is the preferred receptor for NMB (therefore it is also known as NMB receptor) while BB2 is the preferred receptor for GRP.2 BB3 was the third member of the bombesin family of receptors to be cloned based upon its sequence similarity to BB1 and BB2.1 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors, as are its affinities for NMB and GRP. This suggests that the endogenous mammalian ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB1 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.1BB1 is expressed in the brain where it likely mediates the inhibition of food intake and regulates the stress and fear response. BB1 and its ligand NMB are expressed in the pituitary where they participate in the regulation of thyroid-stimulating hormone (TSH) secretion in both autocrine and paracrine fashions.3 In the periphery, BB1 is expressed in the gastrointestinal and urogenital smooth muscle but its physiological function in these tissues is unclear.Finally, BB1 is over-expressed in several human tumors especially colon and non-small lung cancer where it acts as a growth factor receptor inducing tumor growth. BB1 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4

Alternative names: Bombesin Receptor 1 (NMBR), BB1R, Neuromedin B receptor, Neuromedin-B-preferring bombesin receptor

Gene ID: 4829

NCBI Accession: NM_001324307

UniProt: P28336