NPY1R antibody (3rd Intracellular Loop)

Catalog No. ABIN7043371

This Rabbit Polyclonal antibody specifically detects NPY1R in WB, IHC, IF and IC. It exhibits reactivity toward Human.

Quick Overview for NPY1R antibody (3rd Intracellular Loop) (ABIN7043371)

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-NPY1R Antibody

Binding Specificity: 3rd Intracellular Loop, AA 237-252

Purpose: A Rabbit Polyclonal Antibody to Neuropeptide Y1 Receptor

Cross-Reactivity: Human, Rat

Predicted Reactivity: Rat,mouse - 14,16 amino acid residues identical

Characteristics: Anti-NPY1R Antibody is directed against an intracellular epitope of the human neuopeptide Y1 receptor (NPY1R). Anti-NPY1R Antibody (ABIN7043371, ABIN7044668 and ABIN7044669) can be used in western blot analysis, immunohistochemistry, and immunocytochemical applications. It has been designed to recognize NPY1R from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RLKRRNNMMDKMRDNK, corresponding to amino acid residues 237-252 of human NPY1R

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC|ICC

Negative Control: (ABIN7236086)

Blocking Peptide: (ABIN7236086)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NPY1R

Target: NPY1R (Neuropeptide Y Receptor Y1 (NPY1R))

Alternative Name: NPY1R

Background:

Synonyms: Neuropeptide Y1 receptor, Neuropeptide Y receptor type 1, NPYR, NPYY1

Description: Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with the peptide YY (PYY) and the pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R) and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.3 Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2NPY1R is considered to be a postsynaptic receptor. In the CNS, NPY1Rs are concentrated in the cortical areas of the brain, cerebral cortex, olfactory nucleus, hippocampus, hypothalamic nuclei, and thalamic nuclei.4 In addition, this receptor has a bimodal role in the immune system, serving as a strong negative regulator on T cells as well as a key activator of antigen-presenting cell function.5

Gene ID: 4886

NCBI Accession: NM_000909

UniProt: P25929

Pathways: Negative Regulation of Hormone Secretion, Feeding Behaviour