NPY5R antibody (3rd Intracellular Loop)

Catalog No. ABIN7886627

The Rabbit Polyclonal anti-NPY5R antibody is suitable to detect NPY5R in samples from Rat. It has been validated for WB, IHC, IF and IC.

Quick Overview for NPY5R antibody (3rd Intracellular Loop) (ABIN7886627)

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY5R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-NPY5R Antibody

Binding Specificity: 3rd Intracellular Loop, AA 291-306

Purpose: A Rabbit Polyclonal Antibody to Neuropeptide Y5 Receptor

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse,bovine,canis - identical, human - 15,16 amino acid residues identical

Characteristics: Anti-NPY5R Antibody is directed against an epitope of the rat neuropeptide Y5 receptor (NPY5R). Anti-NPY5R Antibody (ABIN7043375 and ABIN7044673) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY5R from rat, human and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: SFIRKHRRRYSKKTAC, corresponding to amino acid residues 291-306 of rat NPY5R

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236102)

Blocking Peptide: (ABIN7236102)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NPY5R

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Alternative Name: NPY5R

Background:

Synonyms: Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5

Description: Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4

Gene ID: 25340

NCBI Accession: NM_006174

UniProt: Q63634

Pathways: Feeding Behaviour