NETO2 antibody (Extracellular)

Catalog No. ABIN7043377

Product Details anti-NETO2 Antibody

Target: NETO2 (Neuropilin (NRP) and Tolloid (TLL)-Like 2 (NETO2))

Binding Specificity: AA 189-202, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NETO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to NETO2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-NETO2 (extracellular) Antibody (ABIN7043377, ABIN7044386 and ABIN7044387)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NETO2 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSSQVEQEEKTKPG, corresponding to amino acid residues 189-202 of rat NETO2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GC046

Blocking Peptide: BLP-GC046

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NETO2

Target: NETO2 (Neuropilin (NRP) and Tolloid (TLL)-Like 2 (NETO2))

Alternative Name: NETO2 (NETO2 Products)

Background: Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, BTCL2,NETO proteins are auxiliary subunits that play a crucial role in the modulation of the biophysical properties of Kainate-type glutamate receptors (KARs). KARs are activated to modulate synaptic transmission, network excitability and synaptogenesis. NETO proteins modulate their activity both in vivo and in vitro mostly by regulating the surface expression and synaptic targeting of KARs in neurons.NETO protein expression has been characterized in the adult brain. NETO1 and NETO2 are highly homologous but have different expression patterns and distinct functional effects. High NETO2 expression levels can be found throughout the mammalian brain1,2.The NETO2 structure reveals a single-pass transmembrane protein that contains an extracellular region with two CUB domains that mediates interactions with KAR subunits. The C-terminus is important for modifying and regulating the synaptic abundance of KARs. The intracellular region of NETO2 can be phosphorylated by various kinases in vitro,which regulates synaptic targeting of KARs3.

Alternative names: NETO2, Neuropilin and tolloid-like protein 2, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, BTCL2

Gene ID: 307757

NCBI Accession: NM_018092

UniProt: C6K2K4