NTSR2 antibody (Intracellular)

Catalog No. ABIN7043384

The Rabbit Polyclonal anti-NTSR2 antibody has been validated for WB, IHC, IF and IC. It is suitable to detect NTSR2 in samples from Rat.

Quick Overview for NTSR2 antibody (Intracellular) (ABIN7043384)

Target: NTSR2 (Neurotensin Receptor 2 (NTSR2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NTSR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-NTSR2 Antibody

Binding Specificity: AA 377-392, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Neurotensin Receptor 2

Specificity: Intracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Mouse - 14,16 amino acid residues identical

Characteristics: Anti-Neurotensin Receptor 2 Antibody is directed against an epitope at the intracellular C-terminal domain of the rat Neurotensin receptor type 2. Anti-Neurotensin Receptor 2 Antibody (ABIN7043384, ABIN7044769 and ABIN7044770) can be used in western blot, immunohistochemical and immunocytochemical applications, and recognizes NTR2 from rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50-1:100

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Cited Application: IHC

Negative Control: (ABIN7236129)

Blocking Peptide: (ABIN7236129)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NTSR2

Target: NTSR2 (Neurotensin Receptor 2 (NTSR2))

Alternative Name: NTSR2

Background: NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor,Neurotensin receptor 2 (NTR2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.NTR2 and NTR1 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1NTR2 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+mobilization. However, the exact signaling mechanisms subsequent to NTR2 activation appear to depend on the species and on the cellular system in which the receptor is studied.NTR2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acid secretion.2The most established physiological role of NTR2 is its role in pain transmission as NTR2-defficient mice showed that NTR2 is the likely mediator of the neurotensin-induced analgesic responses.3

Alternative names: Neurotensin Receptor 2, NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor

Gene ID: 64636

NCBI Accession: NM_012344

UniProt: Q63384