Neurotrophin 3 antibody (Intracellular)

Catalog No. ABIN7043392

This Rabbit Polyclonal antibody specifically detects Neurotrophin 3 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Neurotrophin 3 antibody (Intracellular) (ABIN7043392)

Target: Neurotrophin 3 (NTF3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neurotrophin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Neurotrophin 3 Antibody

Binding Specificity: AA 39-48, AA 177-186, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Neurotrophin 3

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,chicken,Xenopus - identical, cat - 9,10 amino acid residues identical

Characteristics: Anti-Neurotrophin 3 Antibody is directed against an epitope of human Neurotrophin 3. Anti-Neurotrophin 3 Antibody (ABIN7043392, ABIN7044748 and ABIN7044749) can be used in western blot and immunohistochemical applications. It has been designed to recognize from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: GEIKTGNSPV(C), corresponding to amino acid residues 39-48 of mature human NT-3 (residues 177-186 of the NT-3 precursor)

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC

Negative Control: (ABIN7236144)

Blocking Peptide: (ABIN7236144)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neurotrophin 3

Target: Neurotrophin 3 (NTF3)

Alternative Name: NTF3

Background: NTF3, Nerve growth factor 2, NGF2, HDNF,The neurotrophins ("neuro" meaning nerve and "trophe" meaning nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1 The structural hallmark of all neurotrophins is the characteristic arrangement of the disulfide bridges, known as the cysteine knot, which has been found in other growth factors such as PDGF.2 The rat and human forms of neurotrophin-3 (NT-3) are 96 % homologous. NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,3 to serve as an anti-inflammatory factor, to suppress microglial activation,4 to play a critical role in regulating T helper 1/T helper 2 cell balance5 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.6 The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3, and p75NTR, which binds all the neurotrophins.7

Alternative names: Neurotrophin 3 (NT-3), NTF3, Nerve growth factor 2, NGF2, HDNF

Gene ID: 4908

NCBI Accession: NM_001102654

UniProt: P20783

Pathways: RTK Signaling, Neurotrophin Signaling Pathway