OPRD1 antibody (Extracellular)

Catalog No. ABIN7043401

Product Details anti-OPRD1 Antibody

Target: OPRD1 (Opioid Receptor, delta 1 (OPRD1))

Binding Specificity: AA 2-18, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPRD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRD1)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize human samples.

Predicted Reactivity: Rat - 13,17 amino acid residues identical

Characteristics: Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody (ABIN7043401, ABIN7044891 and ABIN7044892)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. The antibody is designed to recognize DOR-1 from mouse and rat samples. It will not recognize DOR-1 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)ELVPSARAELQSSPLVN corresponding to amino acid residues 2-18 of mouse delta-Opioid receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-OR014

Blocking Peptide: BLP-OR014

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for OPRD1

Target: OPRD1 (Opioid Receptor, delta 1 (OPRD1))

Alternative Name: OPRD1 (OPRD1 Products)

Background: DOR-1,Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (μ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor.1 Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic μ, δ, and κ receptors.2The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins.1All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the μ-opioid receptor, whereas μ-, δ-, and κ-receptors participate in the control of pain at the spinal level.3 The opioid receptors also mediate the mood-altering properties of opioids.4 Cross-talk between μ- and δ- opioid receptors was demonstrated when subeffective doses of d-opioid receptors agonists modulated m-mediated analgesia.5The δ receptors are discretely distributed in the central nervous system (CNS), with a prominent gradient of receptor density from high levels in forebrain structures to relatively low levels in most hindbrain regions.2

Alternative names: delta-Opioid Receptor (OPRD1), DOR-1

Gene ID: 18386

NCBI Accession: NM_000911

UniProt: P32300

Pathways: Protein targeting to Nucleus