SLC22A3 antibody (1st Extracellular Loop)

Catalog No. ABIN7886276

This Rabbit Polyclonal antibody specifically detects SLC22A3 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for SLC22A3 antibody (1st Extracellular Loop) (ABIN7886276)

Target: SLC22A3 (OCT3) (Organic Cation Transporter 3 (OCT3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC22A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SLC22A3 Antibody

Binding Specificity: 1st Extracellular Loop, AA 83-96

Purpose: A Rabbit Polyclonal Antibody to Organic Cation Transporter 3

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Rat,mouse - 13,14 amino acid residues identical

Characteristics: Anti-SLC22A3 (extracellular) Antibody (ABIN7043416, ABIN7044223 and ABIN7044224) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Organic cation transporter 3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ERRGQGH(S)HRYLLE, corresponding to amino acid residues 83-96 of rat OCT3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236191)

Blocking Peptide: (ABIN7236191)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SLC22A3

Target: SLC22A3 (OCT3) (Organic Cation Transporter 3 (OCT3))

Alternative Name: SLC22A3

Background:

Synonyms: OCT3, Organic cation transporter 3

Description: Organic cation transporter 3 (SLC22A3) belongs to the SLC22A gene family. Organic cation transporters (OCTs) are membrane transport proteins involved in many metabolic processes and detoxification. The transporters are responsible for the uptake and intracellular inactivation of numerous endogenous and exogenous substrates including norepinephrine, epinephrine, dopamine, histamine and serotonin. They are also responsible for the uptake of a variety of anticancer drugs1,2. OCT structure is suggested to have 12 transmembrane domains and a large hydrophobic cleft capable of accommodating a wide variety of chemical species3.In humans, OCT3 is expressed in many tissues, abundantly in the placenta, heart, liver and skeletal muscle. It is also expressed in astrocytes, intestinal epithelium neurons, glia, ependymal cells, and blood-cerebrospinal fluid barrier in choroid plexus epithelial cells. The protein is also widely distributed in the brain. OCT expression is detected in several cancer cell lines and tumor tissue samples1-3.

Gene ID: 29504

NCBI Accession: NM_021977

UniProt: O88446