Otopetrin 1 antibody (Intracellular Loop)

Catalog No. ABIN7886501

The Rabbit Polyclonal anti-Otopetrin 1 antibody is suitable to detect Otopetrin 1 in samples from Mouse. It has been validated for WB and IHC.

Quick Overview for Otopetrin 1 antibody (Intracellular Loop) (ABIN7886501)

Target: Otopetrin 1 (OTOP1)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Otopetrin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Otopetrin 1 Antibody

Binding Specificity: AA 374-388, Intracellular Loop

Purpose: A Rabbit Polyclonal Antibody to Otopetrin-1 Channel

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-OTOP1 Antibody (ABIN7043417, ABIN7044503 and ABIN7044504) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Otopetrin-1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EKSLDESKNPARKLD, corresponding to amino acid residues 374 - 388 of mouse Otopetrin-1 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236195)

Blocking Peptide: (ABIN7236195)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Otopetrin 1

Target: Otopetrin 1 (OTOP1)

Alternative Name: OTOP1

Background:

Synonyms: Proton Channel OTOP1, Otopetrin-1

Description: Otopetrin-1 (Otop-1) is a proton channel involved in sour taste recognition. It belongs to the Otopetrin proton channel family together with Otopetrins-2 and 31. Otop-1 is a dimeric structure, with each subunit forming 12 transmembrane helices divided into structurally similar N- and C-domains2.Taste recognition is very important because it enables to sample chemicals in the environment prior to digestion and hence, plays an important role in feeding and avoiding intoxication2. The sense of sour taste is caused by acids, and the first step in sour taste transduction is an influx of H+ ions through Otop-1 channel into the type three Taste Receptor Cells (TRCs). This leads to a decrease in intracellular pH and operation of a signaling cascade1-3.Otop-1 is specifically expressed in type three taste cells and exhibits the Zn2+ sensitivity that is a key feature observed in the proton current of sour taste cells4.Otop-1 also has a role in regulating intracellular Ca2+ levels required for the formation of otoconia in the vertebrate inner ear. Otoconia are complex calcium carbonate biominerals required for the sensation of gravity5.

Gene ID: 21906

NCBI Accession: NM_177998

UniProt: Q80VM9