Oxytocin Receptor antibody (C-Term, Intracellular)

Catalog No. ABIN7887191

This Rabbit Polyclonal antibody specifically detects Oxytocin Receptor in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for Oxytocin Receptor antibody (C-Term, Intracellular) (ABIN7887191)

Target: Oxytocin Receptor (OXTR)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oxytocin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: KO Validated

Product Details anti-Oxytocin Receptor Antibody

Binding Specificity: AA 346-358, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Oxytocin Receptor

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-Oxytocin Receptor Antibody is directed against an epitope of the rat Oxytocin receptor. Anti-Oxytocin Receptor Antibody (ABIN7043419, ABIN7045341 and ABIN7045342) can be used in western blot analysis. It has been designed to recognize the oxytocin receptor from rat and mouse samples. The antibody is not recommended for use with human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CSARYLKGSRPGE, corresponding to amino acid residues 346-358 of rat Oxtr

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7236202)

Blocking Peptide: (ABIN7236202)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Oxytocin Receptor

Target: Oxytocin Receptor (OXTR)

Alternative Name: OXTR

Background:

Synonyms: OXTR, OT-R, OTR1

Description: Oxytocin receptor belongs to the class 1 family of G-protein coupled receptors (GPCR) and its role is to regulate reproductive physiology, social and emotional behaviors. Like other members belonging to the GPCR superfamily, it has seven transmembrane domains, extracellular N-terminus and intracellular C-terminal tail1,2.The Oxytocin receptor ligand oxytocin, is a peptide that functions both as a hormone and as a neurotransmitter, and plays an important role in social behavior, estrous cycle, induction of labor and milk ejection, anxiety-related behaviors, food intake, stress-related responses, and pain control. Activation of the receptor leads to the stimulation of phospholipase C by interacting with Gαq/11 in response to ligand binding1.Oxytocin receptor is widely abundant in several brain regions including the basal ganglia, the limbic system, the thalamus, the hypothalamus, the brainstem, and the spinal cord. It is also detected in the uterus, mammary gland, ovary, testis, prostate and kidney.Several studies have suggested that administration of oxytocin in humans has the ability to increase generosity, trust and eye gaze. Oxytocin levels in the plasma relates to parent-child bonding behaviors, feelings of romantic love, trust and empathy. Similarly, Oxytocin receptor knockout mice shows several abnormalities in social and emotional behavior such as increased aggression and deficits in nurturing, positive affection, attachment, sensitivity and social memory2,3.

Gene ID: 25342

NCBI Accession: NM_000916

UniProt: P70536

Pathways: Myometrial Relaxation and Contraction, Feeding Behaviour