Plexin A1 antibody (Extracellular, N-Term)

Catalog No. ABIN7887015

The Rabbit Polyclonal anti-Plexin A1 antibody is suitable to detect Plexin A1 in samples from Human. It has been validated for WB, IHC, FACS, IF and LCI.

Quick Overview for Plexin A1 antibody (Extracellular, N-Term) (ABIN7887015)

Target: Plexin A1 (PLXNA1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plexin A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-Plexin A1 Antibody

Binding Specificity: AA 623-636, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Plexin-A1

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - 13,14 amino acid residues identical

Characteristics: Anti-Plexin-A1 (extracellular) Antibody (ABIN7043435, ABIN7045128 and ABIN7045129) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize PLXNA1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)REVAPITRGQGDQR, corresponding to amino acid residues 623-636 of human PLXNA1

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:600

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236260)

Blocking Peptide: (ABIN7236260)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Plexin A1

Target: Plexin A1 (PLXNA1)

Alternative Name: PLXNA1

Background:

Synonyms: PLXNA1, PLXN1, Semaphorin receptor NOV

Description: The plexin family are transmembrane receptors, comprised four subfamilies: plexin-A, B, C, and D. In humans, three members of the plexin-A subfamily have been isolated: plexin-A1, A2, and A3. Plexin A1 is encoded by the PLXNA1 gene. These receptors are characterized by the presence of a conserved intracellular domain of about 600 amino acids known as the sex and plexins domain. This domain is strikingly conserved across the plexin family and consists of two blocks of high sequence conservation separated by a variable linker. The ectodomains of the plexin-A subfamily possess three repeats of the cysteine cluster/Met-related sequences.Plexins act as signal-transducing components containing transmembrane domain of six Gly residues constituting a GxGGGGG motif.Plexins act as functional receptors for members of the semaphorin family. Together they transmit repulsive axon guidance signals for semaphorins inside the axonal growth cone. The plexins act together with their co-receptors, neuropilins. Plexin-A1, in combination with neuropilins exhibits various functions in axon guidance, organogenesis, angiogenesis, tumorigenesis, and immune regulation1-3.Plexin-A1 also plays an important role in the developmental stages of chick heart by working as a receptor for one of the transmembrane semaphorins. In addition Plexin-A1 expressed in dendritic cells is involved in T-dendritic cell interactions in the immune system4.

Gene ID: 5361

NCBI Accession: NM_032242

UniProt: Q9UIW2

Pathways: Smooth Muscle Cell Migration