Plexin C1 antibody (Extracellular, N-Term)

Catalog No. ABIN7887020

The Rabbit Polyclonal anti-Plexin C1 antibody (ABIN7887020) specifically detects Plexin C1 in WB, IHC, FACS and LCI. The antibody is reactive with Human samples.

Quick Overview for Plexin C1 antibody (Extracellular, N-Term) (ABIN7887020)

Target: Plexin C1 (PLXNC1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Plexin C1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Plexin C1 Antibody

Binding Specificity: AA 860-874, Extracellular, N-Term

Purpose: A Rabbit Polyclonal antibody to Plexin-C1 (extracellular)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical Rat - 14 out of 15 amino acid residues identical

Characteristics: A rabbit polyclonal antibody to Plexin-C1 (extracellular)

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EVKIQKENDNFNISK, corresponding to amino acid residues 860 - 874 of human Plexin-C1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236272)

Blocking Peptide: (ABIN7236272)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Plexin C1

Target: Plexin C1 (PLXNC1)

Alternative Name: PLXNC1

Background:

Synonyms: Virus-Encoded Semaphorin Protein Receptor, VESPR, CD232, PLXNC1

Description: Plexin-C1 also known as Virus-encoded semaphorin protein receptor, is a ~210 kDa type I transmembrane receptor that regulates cytoskeletal remodeling and adhesion in human melanocytes.It belongs to the family of Plexin receptors, these are type I transmembrane proteins (A-D) that share a highly conserved cytoplasmic domain and an intracellular region containing an intrinsic Ras-GTPase activating (GAP) domain. Plexin receptors bind semaphorins: a class of secreted and membrane proteins 1. The natural ligand of Plexin C1 is Semaphorin -7A (#ASR-077).Plexin-C1 is highly expressed in melanocytes, neurons, neutrophils, dendritic cells and pre-osteoblasts and it has a complex effect on migration of melanocytes and melanoma, that depends on the phase of progression of this type of cancer 2,3. It also has additional roles in the partitioning of paraventricular and supraoptic neurons in the hypothalamus, in the development of inflamation and in inhibition of neutrophils phagocytosis 3,4.

Gene ID: 10154

NCBI Accession: NM_005761

UniProt: O60486